| 苹果自交不亲和基因PCR扩增程序优化 |
| |
| 引用本文： | 陈超,于葆杰,李保国.苹果自交不亲和基因PCR扩增程序优化[J].天津农业科学,2009,15(4):24-26. |
| |
| 作者姓名： | 陈超 于葆杰 李保国 |
| |
| 作者单位： | 1. 河北省沧州市林业科学研究所,河北,沧州,061000
2. 河北农业大学,林学院,河北,保定,071000 |
| |
| 摘 要： | 通过研究PCR反应体系(DNA、Primer、Mg2+、dNTP、Taq聚合酶、buffer)及退火温度对苹果自交不亲和基因(S基因)扩增结果影响的分析,构建了适合于苹果自交不亲和基因的PCR扩增程序的优化体系。结果表明:在20μL的反应体系中,模板DNA的最适含量为40ng,dNTP最适浓度为0.25mmol/L,而Primer最适浓度为0.4μmol/L,Mg2+的最适浓度为2.5mmol/L,Taq聚合酶最适浓度为1U,buffer最适浓度为1×buffer,最适退火温度为50℃。
|
| 关 键 词： | 苹果 S基因 PCR体系优化 |
Optimized Study on PCR Amplification Procedure of the Apple Self-incompatibility Gene |
| |
| CHEN Chao,YU Bao-jie,LI Bao-guo.Optimized Study on PCR Amplification Procedure of the Apple Self-incompatibility Gene[J].Tianjin Agricultural Sciences,2009,15(4):24-26. |
| |
| Authors: | CHEN Chao YU Bao-jie LI Bao-guo |
| |
| Institution: | CHEN Chao, YU Bao-jie, LI Bao-guo (1.Forestry Science Institute of Cangzhou, Cangzhou, Hebei 061001, China; 2. Forestry College, Hebei Agricultural University, Baoding, Hebei 071000, China) |
| |
| Abstract: | Through analyzing the effect of PCR system （DNA, Primer, Mg^2＋, dNTP, Taq polymerase, and buffer） and annealing temperature on amplification of apple self-incompatibility gene （s-gene）, we constructed a optimized system of PCR amplification procedure which was fit for apple self-incompatibility gene. The result showed that the optimized content in 20 μL system contained 40 ng DNA template, 0.25 mmol/L dNTP, 0.4 μmol/L primer, 2.5 mmol/L Mg^2＋, 1 U Taq polymerase, lxbuffer, and the optimized annealing temperature was 50 ℃. |
| |
| Keywords: | apple s-gene optimized PCR system |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
