野生卷丹百合黄酮类化合物抗氧化能力的研究

以野生卷丹百合、兰州百合为材料,测定D-101大孔树脂纯化处理前后的总黄酮含量,并比较卷丹百合、芦丁和兰州百合的抗氧化能力。结果表明,用D-101大孔树脂纯化处理前后卷丹百合中总黄酮含量分别为2.187 9和1.723 4 mg/g,兰州百合在纯化前后总黄酮含量分别为1.650 6和1.326 3 mg/g。卷丹百合总黄酮对O2-.、.OH和DPPH都有良好的清除能力且清除效果强于芦丁及兰州百合,表现为:①当提取物质量浓度达到4.00 g/L时,对O2-.的清除率达到89.64%,比同质量浓度芦丁与兰州百合分别高出70.58%和2.57%;②当提取物质量浓度达到2.40 g/L时,对.OH的清除率为62.83%,而同质量浓度芦丁与兰州百合分别为35.68%和25.35%;③当提取物质量浓度达到1.00 g/L时,对DPPH的清除率为47.45%,在同质量浓度下分别比芦丁与兰州百合高出6.82和7.39个百分点。

Antioxidant Ability of Flavonoids from Wild Lilium lancifolium

This paper,taking the wild Lilium lancifolium as the study material,mainly dealt with the antioxidant ability of flavonoids from L.lancifolium after purification by using D-101 macroporous resin,compared to the abilities of Rutin and those from L.davidii var.unicolor.It was shown that approximately 2.187 9 mg/g of total flavonoids were contained in L.lancifolium,and approximately 1.723 4 mg/g after purification,with a recovery percent of 78.77%;the scavenging effects of L.lancifolium against O2-·,·OH and DPPH were evident and stronger than those of Rutin and L.davidii var.unicolor:(i) when purified flavonoids mass concentration got to 4.00 mg/mL,the scavenging rate of L.lancifolium against O2-· peaked at 89.64%,70.58% and 2.57% higher than Rutin and L.davidii var.unicolor at the same mass concentration respectively;(ii) as the concentration went up to 2.40 g/L,the scavenging rate against ·OH reached 62.83%,while 35.68% and 25.35% for Rutin and L.davidii var.unicolor at the same mass concentration respectively;(iii) the scavenging rate against DPPH was 47.45% with the concentration of purified flavonoids of 1.00 g/L,6.82 and 7.39 points more than Rutin and L.davidii var.unicolor at the same concentration respectively.