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Intracellular reactive oxygen species production by polymorphonuclear leukocytes in bovine leukemia virus-infected dairy cows
Authors:Souza Fernando Nogueira de  Blagitz Maiara Garcia  Latorre Andréia Oliveira  Ramos Sanchez Eduardo Milton  Batista Camila Freitas  Weigel Rebeca Alves  Renno Francisco Palma  Sucupira Maria Claudia Araripe  Della Libera Alice Maria Melville Paiva
Affiliation:Department of Clinical Science, Faculty of Veterinary Medicine and Animal Sciences, University of S?o Paulo, Cidade Universitária, S?o Paulo, SP 05508–270, Brazil. nogueirasouza@yahoo.com.br
Abstract:The present study assesses the oxidative burst activity from polymorphonuclear leukocytes (PMNLs) from bovine leukemia virus (BLV)-infected cows. Fifteen clinically healthy cows were divided into serologically positive cows without any hematological alteration, serologically positive animals with persistent lymphocytosis (PL) and healthy serologically negative cows. The oxidative burst activity from the PMNLs was evaluated by flow cytometry using 2',7'-dichlorofluorescein diacetate as a probe. PMNLs from each cow were incubated with heat-killed Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) to stimulate oxidative burst activity. The results of the present work showed no significant difference in the oxidative burst activity without any stimulus and elicited by S. aureus. Conversely, a decrease in the oxidative burst index induced by E. coli in PMNLs was observed in BLV-infected cows.
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