| 84K杨叶片外植体再生系统的建立 |
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| 引用本文： | 樊军锋,李玲,韩一凡,李嘉瑞.84K杨叶片外植体再生系统的建立[J].西北林学院学报,2002,17(2):33-36. |
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| 作者姓名： | 樊军锋 李玲 韩一凡 李嘉瑞 |
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| 作者单位： | 西北农林科技大学,陕西,杨陵,712100; 中国林科院林业研究所,北京,100096 |
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| 基金项目： | 陕西省科技厅科技攻关项目 (2 0 0 1K0 1-G7) |
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| 摘 要： | 利用正交试验等设计,首次系统开展了84K杨叶片外植体再生系统建立研究,确定了84K杨叶片外植体最适不定芽诱导培养基及激素配比为MS+6-BA1.5 mg/L+NAA0.1 mg/L,不定芽最适生根诱导培养基及激素配比为GMS+NAA0.01 mg/L+IBA0.25 mg/L.该实验结果为84K杨工厂化育苗及用叶盘法开展基因转化奠定了良好基础.
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| 关 键 词： | 84K杨 叶外植体 再生系统 |
| 文章编号： | 1001-7461(2002)02-0033-04 |
| 修稿时间： | 2002年4月8日 |
Establishment of Leaf-explant Regeneration System of Poplar 84K |
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| FAN Jun feng ,LI Ling ,HAN Yi fan ,LI Jia rui.Establishment of Leaf-explant Regeneration System of Poplar 84K[J].Journal of Northwest Forestry University,2002,17(2):33-36. |
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| Authors: | FAN Jun feng LI Ling HAN Yi fan LI Jia rui |
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| Institution: | FAN Jun feng 1,LI Ling 2,HAN Yi fan 2,LI Jia rui 1 |
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| Abstract: | Through the application of orthogonal designing, a systematic research into the establishment of the leaf explant regeneration systems for the poplar 84K was for the first time carried out. The optimal differentiation medium and proportion of hormone for leaf explant is MS+BA1.5 mg/L+NAA0.1 mg/L, and the optimal rooting medium and proportion of hormone for shoot is GMS+NAA0.01mg/L+IBA 0.25 mg/L. The results achieved laid a sound foundation for regeneration of poplar 84k on an industrial basis and gene transformation by means of leaf disc method. |
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| Keywords: | poplar 84K leaf explant regeneration system |
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