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| 一种适合AFLP分析的油茶DNA提取方法 | |
| 引用本文： | 黄永芳,廖国春,安星,吴雪辉,阮瑶瑶,谭裕开. 一种适合AFLP分析的油茶DNA提取方法[J]. 广东林业科技, 2009, 25(1): 23-26 |
| 作者姓名： | 黄永芳 廖国春 安星 吴雪辉 阮瑶瑶 谭裕开 |
| 作者单位： | 1. 华南农业大学经济林研究中心,广东广州,510642 2. 广东省肇庆市林业科学研究所 |
| 基金项目： | 广东省自然科学基金项目（06025809），国家“十一五”科技支撑项目（2006BAD18B0204）。 |
| 摘 要： | 以油茶的成熟叶片为材料提取DNA,40个样品用改良的提取纯化方法成功提取出适用于AFLP分析的高质量DNA,OD260/OD280在1.7-2.0之间,可以用于AFLP反应。该方法打破了仅能用幼嫩叶片作为提取材料的限制,具有更广泛的适用性和重要的实用性。 |
| 关 键 词： | 油茶 DNA提取 AFLP反应 |
| 收稿时间： | 2008-11-05 |
| 修稿时间： | 2009-03-09 |
DNA Extraction of AFLP Reaction System for Camellia oleifera |
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| Huang Yongfang,Wu Xue-hui,and. DNA Extraction of AFLP Reaction System for Camellia oleifera[J]. Forestry Science and Technology of Guangdong Province, 2009, 25(1): 23-26 | |
| Authors: | Huang Yongfang Wu Xue-hui and |
| Affiliation: | Huang Yongfang, An Xing,Liao Guochun,Ruan Yaoyao,Wu Xuehui,Tan Yukai(1.Center for No-timber Forest Research, South China Agriculture University, Guangzhou, 510642; 2. Zhaoqing Forestry Research Institute, Guangdong) |
| Abstract: | DNA of samples was extracted and purified from mature leaves, 40 samples were succeed to extracted high quality DNA by using reformative extraction method. OD260/OD280 value is between 1.7 and 2.0, DNA is suitable for AFLP reaction. This method has broken the limitation of using tender leaves only for DNA extraction, which has wider range applicability and great practical significance. |
| Keywords: | Camellia Oleifera Abel DNA extraction AFLP reaction |
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