仙人掌多糖提取的二次优化研究

在以仙人掌多糖提取时间、提取温度和料液比3个因素最佳配合基础上,选取酒精用量、沉淀时间两因素进行二次回归正交组合设计试验,对其提取工艺参数进行优化研究。利用MATLAB7.0软件对仙人掌多糖提取产量的二次多项数学模型解逆矩阵分析表明:在酒精用量为6.52倍,沉淀时间59.7h的条件下,仙人掌多糖提取最大预测值为35.22g/100ml,实际值为35.17g/100ml,两者基本相符。利用优化工艺参数提取仙人掌多糖时,具有最大的提取产量。

Optimization of Extraction Technique of Polysaccharide from Opuntia via Response Surface Methodology

On the basis of the three factors different temperature, time and the ratio of solid to solution on the extraction yield of polysaceharide from Opuntia with the best, the effects of alcohol consumption and Precipitation time were investigated based on composite design of quadratic regression. The extraction technique parameters were optimized Experimental data were analyzed by solving the regression equation with Design MATLAB 7.0 software. It was indicated that the optimum extraction parameters were alcohol consumption 6.52 times ,Precipitation time 59.7 h. Under those conditions, the predicted value of polysaccharidc extraction yield from Opuntia was 35.22 g/100 ml. which was in consistent with the measured value 35.17 g/100ml. It had maximal extraction yield of polysaecharide from Opuntia with optimized technique parameters.