我国部分地方鸡种血浆蛋白质(酶)多态性分析

采用垂直板聚丙烯酰胺凝胶电泳和淀粉凝胶电泳法，检测我国12个地方鸡种淀粉酶、酯酶、运铁蛋白及碱性磷酸酶等4种血浆蛋白质(酶)结构基因座的多态性。12个地方鸡种中所测的4个座位均表现出多态。根据检测座位的基因频率，计算群体平均基因杂合度。并用模糊聚类法对所研究的12个地方鸡种进行聚类。结果表明：泰和乌骨鸡和白耳鸡在λ为0．996时首先聚在一起，然后再与狼山鸡、仙居鸡、河南斗鸡、萧山鸡、固始鸡和藏鸡等依次聚为一大类，表明泰和乌骨鸡和白耳鸡有较近的亲缘关系，而北京油鸡与其他鸡种距离较远。

Studies on the Polymorphisms of Serum Protein (Enzymes) of Chinese Native Chicken Breeds

The polymorphsims of 4 serum protein (enzymes) (Amy-1, Tf, Es-1 and Akp-1) in twelve Chinese native chicken breeds were analysed using vertical polyacrylamide gel electrophesis. The polymorphsim of Akp-1 was examined by zone electorphoresis on starch gels. The results showed that all 4 kinds of loic had polymorphic evedience in 12 Chinese native chicken breeds. Average gene heterozygosity was calculated based on the gene frequency of each locus. A dendrogram was drawn by the fuzzy cluster analysis method. The genetic relationship of 12 Chinese native chicken breeds from close to further was Silkies, Baier chicken, Langshan chicken, Xianju chicken, Games, Xiaoshan chicken, Langshan chicken, Gushi chicken, Tibetan chicken, Chuahua chicken, Dagu chicken, Fatty chicken.