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挤压法生产淀粉糖浆副产物酶解条件
引用本文:肖志刚,申德超,吴谋成,申勋宇.挤压法生产淀粉糖浆副产物酶解条件[J].农业工程学报,2008,24(9):288-293.
作者姓名:肖志刚  申德超  吴谋成  申勋宇
作者单位:1. 东北农业大学食品学院,哈尔滨,150030;华中农业大学食品科学与工程博士后科研流动站,武汉,430070
2. 山东理工大学轻工与农业工程学院,淄博,255049
3. 华中农业大学食品科学与工程博士后科研流动站,武汉,430070
基金项目:黑龙江省科技厅青年科学技术专项,黑龙江省教育厅科学技术研究项目
摘    要:为实现低值蛋白质资源玉米渣的高质化利用,采用2709碱性蛋白酶,水解挤压法生产淀粉糖浆的副产物玉米渣,确定水解工艺条件.并对水解液中的肽分子质量分布状况及氨基酸组成进行了分析.结果表明,按照本研究工艺路线,采用2709碱性蛋白酶能够较为彻底地水解目标副产物中的蛋白质,水解液中分子质量小于10000 u的水解物大于总量的94.8%,并且绝大部分水解物在二肽、三肽分子质量范围内分布,水解液中含有能够满足人体生理机能需要的多种氨基酸.

关 键 词:挤压  分子质量  淀粉  糖浆  酶解液
收稿时间:2007/8/10 0:00:00
修稿时间:2008/9/22 0:00:00

Enzyme hydrolysis of starch syrup by-product with extrusion technology
Xiao Zhigang,Shen Dechao,Wu Moucheng and Shen Xunyu.Enzyme hydrolysis of starch syrup by-product with extrusion technology[J].Transactions of the Chinese Society of Agricultural Engineering,2008,24(9):288-293.
Authors:Xiao Zhigang  Shen Dechao  Wu Moucheng and Shen Xunyu
Institution:1. College of Food Science, Northeast Agricultural University, Harbin 150030, China; 2. Postdoctoral Scientific Research Mobile Station of Food Science and Engineering, Huazhong Agricultural University, Wuhan 430070, China,School of Light Industry and Agricultural Engineering, Shandong University of Technology, Zibo 255049, China,Postdoctoral Scientific Research Mobile Station of Food Science and Engineering, Huazhong Agricultural University, Wuhan 430070, China and School of Light Industry and Agricultural Engineering, Shandong University of Technology, Zibo 255049, China
Abstract:To realize the comprehensive utilization of corn residue, the corn residue of starch syrup by-product with extrusion technology was hydrolyzed by the Alkaline Protease 2709. Gel column filtration chromatography and automatic amino acide analyzer were used to determine the molecular distribution, amino acid compositions and contents in the hydrolysate. The results showed that protein in by-product can better be hydrolyzed by the Alkaline Protease 2709, the products with molecular weight(Mw) below 1000 u in hydrolysate was over 94.8%, and most of hydrolysate are dipeptides or tripeptides. The enzyme hydrolysate contains many amino acids necessary for human being.
Keywords:extrusion  molecular weight  starch  syrup  enzyme hydrolysate
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