| 利用改进的Oligo-Capping法构建甘蔗茎全长cDNA文库 |
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| 引用本文： | 郭晋隆,阙友雄,刘金仙,郑益凤,陈如凯,许莉萍.利用改进的Oligo-Capping法构建甘蔗茎全长cDNA文库[J].热带作物学报,2009,30(5):672-676. |
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| 作者姓名： | 郭晋隆 阙友雄 刘金仙 郑益凤 陈如凯 许莉萍 |
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| 作者单位： | 福建农林大学,农业部甘蔗遗传改良重点开放实验室,福州,350002 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，福建省自然科学基金，引进国际先进农业科学技术计划(948计划)，福建省科技厅国家科技项目 |
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| 摘 要： | Oligo-Capping法是目前全长cDNA文库构建的重要方法之一.笔者在引进、消化和吸收的基础上,通过对特异引物和接头序列的设计,及cDNA扩增反应条件的优化,对该方法进行了一些切实可行的改进,形成了一套简便易行的技术体系.利用本研究中改进的Oligo-Capping法,成功地构建了甘蔗茎全长cDNA文库.综合评价结果表明,本研究中所构建的甘蔗茎全长cDNA文库的库容大于2.5x106cfu,重组率为91%,全长率高达88.9%.本文库的构建为进一步甘蔗茎中全长基因的克隆和功能基因的表达分析奠定了基础.
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| 关 键 词： | 全长cDNA文库 Oligo-Capping法 甘蔗茎 |
Construction of Full-length cDNA Library for Sugarcane Stemby Optimized Oligo-capping |
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| Guo Jinlong,Que Youxiong,Liu Jinxian,Zheng Yifeng,Chen Ruka and Xu Liping.Construction of Full-length cDNA Library for Sugarcane Stemby Optimized Oligo-capping[J].Chinese Journal of Tropical Crops,2009,30(5):672-676. |
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| Authors: | Guo Jinlong Que Youxiong Liu Jinxian Zheng Yifeng Chen Ruka and Xu Liping |
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| Institution: | Guo Jinlong,Que Youxiong,Liu Jinxian,Zheng Yifeng,Chen Ruka,Xu Liping Ministry of Agriculture Key Laboratory for Sugarcane Genetic Improvement,Fujian Agriculture , Forestry University,Fuzhou,Fujian 350002,China |
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| Abstract: | Oligo-capping is one of the most important methods in the construction of full-length cDNA library. In order to discover new genes in a throughput manner, the Oligo-capping method published previously was modified to raise the efficiency in the construction of full-length cDNA library, and a quick and simple technical system for constructing a full-length cDNA library was then successfully established. Using the optimized protocol, we successfully constructed a full-length cDNA library for sugarcane stem, w... |
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| Keywords: | full-length cDNA library Oligo-capping sugarcane stem |
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