茶树扦插苗圃土壤真菌群落结构多样性及其环境因子分析

扦插土壤微生物在茶树扦插短穗愈合生根过程中起着至关重要的作用。为探究不同类型苗圃土壤之间的真菌群落结构差异以及土壤环境因子与真菌群落结构之间的关系。本实验采用高通量测序技术对5个茶区苗圃地的非重茬表土、重茬表土、黄心土样品进行测序分析,比较不同类型扦插土壤中真菌群落多样性差异。Alpha多样性分析表明,黄心土的真菌群落的总数和多样性都低于重茬表土和非重茬表土,重茬表土和非重茬表土的真菌群落总数和多样性在不同地区存在差异。从五组土壤样品中共检测出5个门,其中优势菌群为接合菌门(Zygomycota),丰度占真菌群落的31.11%~77.00%。五组土壤样品的403个真菌菌属中,优势菌属均为未被分类的被孢霉科(unclassified_Mortierellaceae)。相关性分析表明,真菌OTU总数和物种总数Chao1与土壤有机质含量SOM有极强的相关性。本研究结果为茶树扦插土壤的选择提供了参考,也为茶树扦插土壤中病原微生物的进一步的筛选分离等研究提供了科学依据。

Diversity of Soil Fungal Community Structure and Its Driving Factors in Tea Plant Cuttings Seedling Nursery

Soil microorganisms play an important role in the healing and rooting of tea plant cuttings. In order to explore the difference of fungal community structure between different types of nursery soils and the relationship between soil environmental factors and fungal community structure. High-throughput sequencing technology was used to compare and analyze the differences of three different cutting soils, noncontinuous cropping topsoil,continuous cropping topsoil and yellow soil, as well as the fungal community structure between different regions.Alpha diversity analysis showed that the total number and diversity of fungal communities in yellow soil were lower than that in continuous cropping topsoil and noncontinuous cropping topsoil, and the total number of species were different in different regions. Five fungal phyla were detected from 5 group soil samples, Zygomycota was the dominant microflora, accounting for 31.11%~77.00% of the fungal community. Among the 403 fungal genera in the 5 groups of soil samples, the dominant genus was all unclassified_Mortierellaceae. Correlation analysis showed that the total number of OTU and Chao1 are highly correlated with SOM. This study provides a reference for the selection of tea plant cuttage soil, and provides a scientific basis for further screening and isolation of pathogenic microorganisms in tea plant cuttage soil.