拟南芥茉莉酸信号途径突变体jar1的灰葡萄孢菌抗性分析

为研究拟南芥JAR1(At2g46370)基因在灰葡萄孢菌(Botrytis cinerea)抗性反应中的作用,从拟南芥生物资源中心获得其功能下降突变体jar1(SALK_030821)。以纯合突变体为材料,将灰葡萄孢菌的孢子悬浮液接种到4周龄的拟南芥叶片上,统计接种后一周内植株病情指数。通过实时荧光定量RT-PCR测定灰葡萄孢菌肌动蛋白基因(B.cinerea actin)在拟南芥叶片中的表达量,分析了病原物的繁殖情况。结果发现,从接种后的第2天开始,jar1植株的病情指数显著高于野生型。qRT-PCR检测结果表明,在接种后的第三天,野生型植株中B.cinerea actin的表达量是对照的6.9倍,而jar1植株中B.cinerea actin的表达量是对照的20.2倍,说明病原物在jar1突变体叶片上的繁殖速度显著高于在野生型中的。本研究的结果初步表明JAR1基因参与拟南芥对灰葡萄孢菌的抗性调控。

Resistant Analysis of Jasmonic Acid Signaling Mutant jar1 to Botrytis cinerea in Arabidopsis

In order to study the role of JAR1(At246370)gene of Arabidopsis thaliana in response to Botrytis cinerea,jar1(SALK_030821),a loss function mutant of JAR1 was obtained from the Arabidopsis Biological Resource Center.Homozygous mutants were used as materials,the spore suspension of B.cinerea was inoculated on the leaves of homozygous jar1 mutants grown for 4 weeks.The disease index of leaves were estimated within one week after inoculation.The growth of fungus on the leaves,reference to the actin gene expression of B.cinerea actin,was analyzed by qRT-PCR.The results showed that the disease index of jar1 leaves was significantly higher than that in wild-type after inoculation with the pathogen from the second day.The results of qRT-PCR showed that the expression of B.cinerea actin in wild type plants was 6.9 times higher than that in the control,while the expression of B.cinerea actin in jar1 plants was 20.2 times higher than that in the control on the third day after inoculation.It is indicated that the propagation speed of the pathogen on the jar1 mutant leaves is significantly higher than in the wild-type.The results of this study preliminarily indicated that JAR1 gene was involved in the resistance regulation of A.thaliana to B.cinereal.