SSR markers reveal the uniqueness of olive cultivars from the Italian region of Liguria |
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Authors: | Tania Bracci Luca Sebastiani Matteo Busconi Corrado Fogher Angjelina Belaj Isabel Trujillo |
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Affiliation: | 1. Scuola Superiore Sant’Anna, Piazza Martiri della Libertà 33, 56127 Pisa, Italy;2. Istituto di Botanica e di Genetica Vegetale, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29100 Piacenza, Italy;3. IFAPA, Centro “Alameda del Obispo”, Avenida Menéndez Pidal s/n, 14004 Córdoba, Spain;4. Departamento de Agronomia, Universidad de Córdoba, Campus Universitario de Rabanales, Edificio Celestino Mutis, Ctra Madrid-Cadiz km 396, 14004 Córdoba, Spain |
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Abstract: | Twenty-three important Ligurian olive accessions corresponding to 16 cultivars were studied using 12 SSR markers and 40 Mediterranean cultivars were included in the study in order to investigate the relationships between Ligurian and Mediterranean germplasm. All SSRs produced polymorphic amplifications. One hundred and forty-nine alleles were found in the 63 accessions analysed. Twenty-two alleles were specific to germplasm from Liguria and of these 12 were unique to single cultivars. Heterozygosity and discriminating power calculated in this regional germplasm were high on average (0.70 and 0.74) and not so much lower than the values in the total sample that includes cultivars from different Mediterranean countries (0.77 and 0.88 respectively). No cases of genetic identities were found between Ligurian and Mediterranean accessions. Several cases of homonyms and synonyms within the Ligurian germplasm were explained. Cluster analysis generally revealed a clear discrimination of the profiles from Liguria and Italy with respect to the cultivars from other Mediterranean countries. Only one Ligurian cultivar, “Negrea”, appeared to have a different origin, grouping with the Mediterranean cultivars. |
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Keywords: | Olea europaea L. Germplasm conservation Genetic diversity Identification Microsatellites |
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