葡萄胚珠诱导成苗的研究

通过对葡萄几个品种胚珠愈伤组织的诱导、再分化,以胚状体发生途径实现植株再生。结果表明:以葡萄的胚珠为外植体,在附加不同浓度的2,4-D与6-BA的MS、B5和NN-1969培养基上培养,愈伤组织诱导以NN+2,4-D0.5mg/L+6-BA1.0mg/L培养基最佳,诱导率为84.1%,胚性愈伤组织诱导率为79.5%;将胚性愈伤组织置于含NAA0.03mg/L和6-BA0.5mg/L的NN培养基中,胚状体诱导率为6%～15%;胚状体在无激素MS培养基中的成苗率为0%～20%。

Study on the induction and plantlet regeneration from ovule of grape

Calli which were induced from ovule of several cultivars of grape were differentiated and regenerated into plantlets by the way of embryogenesis.The results showed as follows: when ovule explants of these cultivars were cultured on MS (Murashige and Skoog 1962),B5(Gamborg 1968) and NN(Nitsch and Nitsch 1969) medium supplied with 2,4-D and 6-BA in combination, the medium NN+2,4-D 0.5mg/L +6-BA 1.0 mg/L was the best for inducing calli, the rate of calli induction was 84.1 %, and the rate of embryogenic calli induction was 79.5 %; when embryogenic calli were transferred to NN medium containing NAA 0.03 mg/L and 6-BA 0.5 mg/L for embryoid induction, the inductiverate of embryoids was 6 %～15 %; when embryoids were transferred into MS medium without any growth regulators the ratio of embryoid regeneration ranged from 0 % to 20 %.