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桑叶叶绿素光谱特征及其含量测定的初步研究
引用本文:邓祥元,高坤,张丽娟.桑叶叶绿素光谱特征及其含量测定的初步研究[J].安徽农业科学,2010,38(31):17423-17424,17426.
作者姓名:邓祥元  高坤  张丽娟
作者单位:江苏科技大学生物与环境工程学院,江苏镇江,212018;江苏科技大学生物与环境工程学院,江苏镇江,212018;江苏科技大学生物与环境工程学院,江苏镇江,212018
基金项目:江苏科技大学引进人才科研启动基金(35290902)
摘    要:目的]研究桑叶叶绿素的光谱特征及其含量的变化规律。方法]以新鲜桑叶为材料,采用有机溶剂萃取法和分光光度法对桑叶叶绿素进行提取与含量测定,通过比较不同节位桑叶及桑叶不同部位的叶绿素含量,分析桑叶叶绿素的光谱特征及其含量的变化规律。结果]桑叶叶绿素的光谱特征与其他高等植物叶绿素的光谱特征类似;不同节位桑叶中叶绿素含量为中部〉底部〉顶端,但叶绿素a/b的比值却为底部〉硕端〉中部;而桑叶不同部位叶绿素含量及a/b比值的高低顺序依次为叶柄〉叶中〉叶尖。结论]不仅为叶绿素的提取及含量测定提供了方法性参考,而且有益于叶绿素的进一步研究。

关 键 词:叶绿素  光谱特征  分光光度法  桑叶

Preliminary Study on Spectral Characteristics and Content of Chlorophyll in Mulberry Leaves
DENG Xiang-yuan et al.Preliminary Study on Spectral Characteristics and Content of Chlorophyll in Mulberry Leaves[J].Journal of Anhui Agricultural Sciences,2010,38(31):17423-17424,17426.
Authors:DENG Xiang-yuan
Institution:DENG Xiang-yuan et al(College of Biotechnology , Environmental Engineering,Jiangsu University of Science , Technology,Zhenjiang,Jiangsu 212018)
Abstract:Objective] The aim was to further explore spectral characteristics and variance of chlorophyll content in mulberry leaves.Method] Fresh mulberry leaves were used as raw material in this study,and chlorophyll was extracted by organic solvent extraction process and measured with ultraviolet-visible spectrophotometry.Chlorophyll contents in different node order and section of mulberry leaves were compared and analyzed to obtain spectral characteristics and Variance of chlorophyll content.Result] It was foun...
Keywords:Chlorophyll  Spectral characteristics  Spectrophotometry  Mulberry leaves  
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