蛀虫蜡蚧菌的产孢条件优化

蛀虫蜡蚧菌Lecanicillium cauligalbarum是蜡蚧菌属中最近报道的新种，目前对其研究尚属空白。为寻求易于生长的培养基成分，本文通过单因素筛选最佳碳源、氮源、生长因子并进行正交试验优化。筛选的产孢条件（m/v）为：可溶性淀粉1%，酵母粉3%，蚕蛹粉1.5%，基础培养基（氯化钾0.05%，磷酸氢二钾0.1%，七水硫酸镁0.05%，七水硫酸亚铁0.001%），琼脂1.5%，蒸馏水1000 mL，25℃。优化培养基产孢量是PDA的4.5倍，是沙氏培养基的15.6倍。结果还显示该菌的产孢量受氮源影响较大，并对无机氮利用较低。这些结果将为蛀虫蜡蚧菌的后续研究提供基础资料。

Optimization of Spore-producing Conditions for Lecanicillium cauligalbarum

Lecanicillium cauligalbarum is a new species reported in the genus of Lecanicillium, research about it is scarcely. To establish the medium components for culturing this fungus, the optimal carbon source, nitrogen source, and growth factors were screened individually and orthogonal experiments were conducted to screen the best spore-producing conditions. The optimal conditions for sporulation (mass to volume ratio) were:soluble starch 1%, yeast powder 3%, silkworm chrysalis powder 1.5%, potassium chloride 0.05%, dipotassium phosphate 0.1%, magnesium sulfate 0.05%, ferrous sulfate 0.001%, agar 1.5%, distilled water 1000 mL, and temperature of 25℃. L. cauligalbarum was greatly influenced by the nitrogen source but absorbed only low inorganic nitrogen. The spore yield in the optimized medium was 4.5 times of that of PDA and 15.6 times of that of SAB medium. The results provide basic data for the follow-up research on L. cauligalbarum.