| 湖北省现行家蚕品种原原种AFLP指纹图谱的构建 |
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| 引用本文： | 吴凡,李德臣,赵春晓,陈登松.湖北省现行家蚕品种原原种AFLP指纹图谱的构建[J].北方蚕业,2015(2):4-8. |
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| 作者姓名： | 吴凡 李德臣 赵春晓 陈登松 |
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| 作者单位： | 1. 湖北省农业科学院经济作物研究所;2. 安康市蚕桑研究所,陕西安康,725019;3. 湖北省农业科学院农业质量标准与检测技术研究所,武汉,430070 |
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| 摘 要： | 利用AFLP分子标记技术,构建了湖北省10个优质强健原原种的AFLP指纹图谱,分析了其遗传相似系数及亲缘关系。利用4对AFLP引物,共扩增了584条带,其中有多态性带354条,多态性比例为60.62%,构建了其AFLP指纹图谱,可以在分子水平上对这10个家蚕原原种进行鉴定和检测。
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| 关 键 词： | 家蚕 A FL P 指纹图谱 品种鉴定 |
The Construction of AFLP Fingerprints among Stocks of Current Silkworm (Bombyx mori) Varieties in Hubei Province |
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| WU Fan;LI Dechen;Zhao Chunxiao;CHEN Dengsong.The Construction of AFLP Fingerprints among Stocks of Current Silkworm (Bombyx mori) Varieties in Hubei Province[J].North Sericulture,2015(2):4-8. |
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| Authors: | WU Fan;LI Dechen;Zhao Chunxiao;CHEN Dengsong |
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| Institution: | WU Fan;LI Dechen;Zhao Chunxiao;CHEN Dengsong;Industrial Crops Institute,Hubei Academy of Agricultural Science;Ankang Sericulture Institute;Institute of Agricultural Quality Standards and Testing Technology,Hubei Academy of Agricultural Science; |
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| Abstract: | The analysis on genetic diversity and phylogenetic relationship among 10 Bombyx mori(silkworm)varieties cur‐rently used in Hubei province was carried out based on AFLP molecular markers .4 primer-pair of AFLP were screened out ,and 584 nucleotide fragments were amplified among which 354 polymorphism nucleotides were found ,with a poly‐morphism rate about 60 .62% .The AFLP fingerprints were conducted ,which can be used to identify 10 Bombyx mori va‐rieties .The results provide a theoretical basis for the protection of silkworm varieties . |
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| Keywords: | silkworm AFLP fingerprint variety identification |
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