激素对小麦幼胚胚性无性系高频率诱导的影响

研究了 3种外源激素对 3个小麦品种 (系 )幼胚体细胞胚性无性系高频率形成的影响。结果表明 :2 ,4- D能显著促进小麦幼胚体细胞胚性无性系的形成 ,并存在着基因型间的差异 ,在小麦体细胞无性系形成中起主要作用。其最佳适用质量浓度为 2 .0～ 3.0 m g/ L ,2 ,4- D的这种作用能被培养基中加入的细胞分裂素所加强 ,这种作用在不同的细胞分裂素间是有差异的 ,在质量浓度 0 .1～ 2 .0 m g· L- 1 内 ,随 KT浓度的升高 ,6 - BA浓度的降低 ,胚性愈伤诱导率提高

Effect of exogenous hormones on high frequency induction of embryogenic somaclone derived from immature embryos of wheat (Triticum aestivum L.)

Effect of three exogenous hormones on high frequency induction of three varieties of wheat (Triticum aestivum L.) immature embryos is studied.The following results are acquired.Concentration of 2,4-D in media significantly affects the induction of embryogenic calli derived from immature embryos of wheat,with different frequency between the three genotypes.The most appropriate concentration of 2,4-Dis at 2.0-3.0 mg/L.This capability of 2,4-D can be enforced by the addition of KT or 6-BA in medium.But this enforcement differs between KT and 6-BA.KT has a significant role in the induction of embryogenic calli at relatively high concentration while 6-BA works well at lower concentration.