| 剑麻的组织培养和快速繁殖 |
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| 引用本文： | 陈伟 徐立 李志英 李克烈. 剑麻的组织培养和快速繁殖[J]. 热带农业科学, 2006, 26(3): 22-24 |
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| 作者姓名： | 陈伟 徐立 李志英 李克烈 |
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| 作者单位： | 中国热带农业科学院热带作物品种资源研究所 海南儋州571737 |
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| 摘 要： | 以剑麻的嫩芽为外植体,在N6 2,4-D2mg/L 6-BA2mg/L NAA0.5mg/L培养基上诱导出愈伤组织,再在MS 6-BA2mg/L NAA0.05mg/L培养基上诱导不定芽和增殖培养,不定芽在MS培养基上诱导生根形成完整植株,生根率达100%。将小苗移栽至基质配比为椰糠∶河沙等于1∶1的育苗盆中,成活率达100%。
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| 关 键 词： | 剑麻 组织培养 快速繁殖 |
| 收稿时间： | 2005-10-13 |
| 修稿时间： | 2005-10-13 |
Tissue Culture and Rapid Propagation of Agave sisalana |
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| CHEN Wei XU Li LI ZhiYing LI KeLie. Tissue Culture and Rapid Propagation of Agave sisalana[J]. Chinese Journal of Tropical Agriculture, 2006, 26(3): 22-24 |
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| Authors: | CHEN Wei XU Li LI ZhiYing LI KeLie |
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| Affiliation: | Tropical Crops Genetic Resources Institute, CATAS, Danzhou 571737, Hainan |
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| Abstract: | Calli were induced on N6 medium supplemented with 2,4-D 2.0 mg/L, 6-BA 2.0 mg/L and NAA 0.5 mg/L by using auxiliary buds of Agave sisalana Perrine ex Engelmann as explants. Adventitious buds were induced and proliferated on the MS at the presence of 6-BA 2.0 mg/L NAA 0.05 mg/L. The adventitious buds cultured on the MS medium were induced to root to form rooted plantlets with the rooting rate being 100%. The rooted plantlets survived by 100% when transplanted onto containers for culture on the medium containing coir and sand at a ratio of 1:1. |
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| Keywords: | Agave sisalana Perr. ex Engelm. tissue culture rapid propagation |
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