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| 山西不同品种大豆贮藏蛋白提取及亚基分析 | |
| 引用本文： | 陈振家,郝利平. 山西不同品种大豆贮藏蛋白提取及亚基分析[J]. 山西农业大学学报(自然科学版), 2007, 27(1): 75-78 |
| 作者姓名： | 陈振家 郝利平 |
| 作者单位： | 山西农业大学,食品科学与工程技术学院,山西,太谷,030801 |
| 摘 要： | 用不同盐缓冲溶液分别提取大豆贮藏蛋白,对提取效果分析比较,结果表明,Tris-HCl(pH 7.8)缓冲溶液的提取方法最为合适;在确定提取方法的基础上,对山西不同品种大豆的贮藏蛋白进行了SDS-PAGE分析,电泳图谱显示,不同品种间贮藏蛋白亚基在数量和含量上有较大差别。 |
| 关 键 词： | 大豆 贮藏蛋白 亚基 SDS-PAGE |
| 文章编号： | 1671-8151(2007)01-0075-04 |
| 修稿时间： | 2006-05-15 |
The Extraction and Subunit Analysis of Storage Protein of Different Soybean Cultivars in Shanxi Province |
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| CHEN Zhen-jia,HAO LI-ping. The Extraction and Subunit Analysis of Storage Protein of Different Soybean Cultivars in Shanxi Province[J]. Journal of Shanxi Agricultural University(Nature Science Edition), 2007, 27(1): 75-78 | |
| Authors: | CHEN Zhen-jia HAO LI-ping |
| Abstract: | To study the subunit of soybean storage protein,storage protein extracted by different buffer from defatted soybean were analyzed by SDS-PAGE electrophoresis.The results showed that duilt Tris-HCl(pH7.8) buffer was the best choice to extract storage protein.Storage protein of different soybean cultivars in Shanxi province were analyzed by SDS-PAGE electrophoresis,SDS-PAGE Patten show that there have differences in the number and content of subunit. |
| Keywords: | SDS-PAGE |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |