HPLC法测定不同产地甘草中甘草苷的含量

[目的]建立甘草中甘草苷含量测定的HPLC法,测定不同产地甘草中甘草苷的含量,以控制其质量。[方法]HPLC色谱条件为：分析柱为Kromasil C18柱（4.6 mm×25 cm,5μm）;流动相为乙腈-0.5%醋酸溶液（V/,V 1∶4）;流速为1.0 ml/min;柱温为25℃;紫外检测波长为270 nm;采用外标法定量测定。[结果]甘草苷在0.1~1.2μg范围内呈良好的线形关系（r=0.999 9）,其平均加样回收率和RSD值分别为97.86%和2.04%（n=6）。[结论]该方法操作简单、准确、快速、重现性好,可作为甘草苷的定量分析方法。

Deterntination of Contents of Liquiritin in RADIX RHIZOMA GLYCYRRHIZAE from Different Producing Areas by HPLC

[Objective] To establish a method for determining contents of liquiritin in RADIX RHIZOMA GLYCYRRHIZAE from different producing areas by HPLC,so as to set up a sensitive and specific method for controlling its quality.[Method] The HPLC chromatographic conditions were as follows: column of Kromasil C18(4.6 mm×25 cm,5 μm),mobile phase of acetonitrile-0.5% acetic acid(V/V,1∶4);flow rate of 1.0 ml/min,column temperature of 25 ℃;detection wavelength of 270 nm;external standard method was adopted in quantitative determination.[Results] The liquiritin presented a good linear relation with peak areas in a range of 0.1 to 1.2 μg(r=0.999 9),whose average recovery and RSD were 97.86% and 2.04%(n=6),respectively.[Conclusion] The method established in this study was simple,convenient and rapid with good repeatability which can be used in quantitative analysis of RADIX RHIZOMA GLYCYRRHIZAE.