水稻OsOle1基因的克隆及其遗传转化 |
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引用本文: | 姚清国,李晓芹,李晓兵,段书德.水稻OsOle1基因的克隆及其遗传转化[J].安徽农业科学,2011,39(21):12641-12642. |
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作者姓名: | 姚清国 李晓芹 李晓兵 段书德 |
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作者单位: | 石家庄学院化工学院,河北石家庄,050035 |
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摘 要: | 目的]克隆水稻OsOle1基因并对其进行遗传转化。方法]通过RT-PCR方法对OsOle1基因进行克隆,将克隆出的OsOle1基因连接到pCAMBIA 1300上构建其超表达载体,并通过农杆菌介导对水稻愈伤进行了遗传转化。结果]克隆出的OsOle1基因全长498bp,编码165个氨基酸,成功构建了其超表达载体Ub::OsOe1-GUS,最终得到了转基因株系。结论]为OsOle1基因的功能研究奠定了基础。
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关 键 词: | 水稻 基因克隆 遗传转化 |
Cloning and Genetic Transformation of OsOle1 Gene in Rice |
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Institution: | YAO Qing-guo et al(Chemistry Department of Shijiazhuang University,Shijiazhuang,Hebei 050035) |
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Abstract: | Objective] The cloning and transformation of rice OsOle1 gene were conducted in the research.Method] OsOle1 gene was cloned by RT-PCR.The amplified OsOle1 was then ligased to pCAMBIA 1300 to construct GUS overexpression vector.Then the Agrobacteriummediated method was used in rice callus transformation.Result]The full-length of OsOle1 gene was 498 bp and it encoded 189 amino acids.The over-expression vector Ub∷OsOe1-GUS was prepared and transgenic plants were successfully obtained.Conclusion]The transgenic lines laid the foundation for the function research of OsOle1. |
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Keywords: | Rice Gene cloning Genetic transformation |
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