生豆浆基因组DNA不同提取方法的比较研究

[目的]探讨适宜生豆浆的基因组DNA提取方法。[方法]以市售豆浆为材料,分别采用热解法、异丙醇沉淀法、CTAB法、SDS法、高盐低pH和异硫氰酸胍法以及它们的改良方法提取基因组DNA,并比较了以上方法的提取效果。[结果]除异丙醇沉淀法外,其他方法提取的基因组DNA均可满足PCR检测要求。同时,综合考虑基因组DNA的纯度和浓度,生豆浆基因组DNA提取方法的优劣依次为：改进高盐低pH法、高盐低pH法、改进CTAB法、改进异丙醇沉淀法、异硫氰酸胍法和改进热解法。[结论]这几种生豆粉基因组DNA提取方法均具有操作简单、耗时短、利于快速检测的优点。

Comparison of Different Extraction Methods of Genomic DNA of Raw Soybean Milk

[Objective] The paper was to explore DNA extraction method of raw soybean milk.[Method] With soybean milk purchased from market as material,pyrolysis method,isopropanol precipitation method,CTAB method,SDS method,high-salt low-pH and guanidine isothiocyanate method,as well as their improve methods were used to extract genomic DNA,and the extraction effects of these methods were compared.[Result] In addition to isopropanol precipitation method,genomic DNA extracted by the rest methods all could meet the needs of PCR detection.Meanwhile,based on the comprehensive consideration of concentration and purity of DNA,the better extraction methods for genomic DNA of raw soybean milk successively were improved high-salt low-pH method,high-salt low-pH method,improved CTAB method,improved isopropanol precipitation method,guanidine isothiocyanate method and improved pyrolysis method.[Conclusion] These extraction methods for genomic DNA of raw soybean milk had simply operation and short time consumption,which were conducive for fast detection.