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| 温氏附红细胞体16S rRNA基因的序列测定和分析 | |
| 引用本文： | 付媛,李树清,杜凯,刘琴,周艳琴,刘恩勇,姚宝安,赵俊龙. 温氏附红细胞体16S rRNA基因的序列测定和分析[J]. 畜牧兽医学报, 2006, 37(5): 518-522 |
| 作者姓名： | 付媛 李树清 杜凯 刘琴 周艳琴 刘恩勇 姚宝安 赵俊龙 |
| 作者单位： | 1. 华中农业大学农业微生物学国家重点实验室,武汉,430070;华中农业大学动物医学院,武汉,430070;浙江省农科院畜牧兽医研究所,杭州,310021 2. 上海进出口检验检疫局,上海,200135 3. 华中农业大学农业微生物学国家重点实验室,武汉,430070;华中农业大学动物医学院,武汉,430070 4. 华中农业大学动物医学院,武汉,430070 5. 湖北省兽疫防治站,武汉,430064 |
| 基金项目： | 湖北省畜牧局课题和上海市科委标准专项（04DZ05017） |
| 摘 要： | 从自然感染附红细胞体的湖北黄牛无菌采集血液,分离附红细胞体并提取病原基因组,用血营养菌的16SrRNA基因的通用引物进行PCR扩增,得到长约1.5kb的扩增片段,将其克隆到pMD18-T载体后进行测序和分析.结果表明该片段全长为1 471 bp（GenBank收录号为AY946266）,同源性分析表明该序列与Neimark公布的温氏附红细胞体（AF016546）的16S rRNA基因序列同源性达到98.7%,证实该病原为温氏附红细胞体,从分子生物学水平证实了温氏附红细胞体在湖北省的存在.将该序列与5种支原体、14种血营养菌及边缘无浆体等的相应序列进行比较,建立系统发育树,结果表明温氏附红细胞体同边缘无浆体的关系较远,而与肺炎支原体组的亲缘关系较近. |
| 关 键 词： | 温氏附红细胞体 16S rRNA PCR 序列分析 |
| 文章编号： | 0366-6964（2006）05-0518-05 |
| 收稿时间： | 2005-04-26 |
| 修稿时间： | 2005-04-26 |
Sequence and Molecular Characterization of 16S rRNA Gene of Eperythrozoon wen yonii-Hubei Strain |
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| FU Yuan,LI Shu-qing,DU Kai,LIU Qin,ZHOU Yan-qin,LIU En-yong,YAO Bao-an,ZHAO Jun-long. Sequence and Molecular Characterization of 16S rRNA Gene of Eperythrozoon wen yonii-Hubei Strain[J]. Chinese Journal of Animal and Veterinary Sciences, 2006, 37(5): 518-522 | |
| Authors: | FU Yuan LI Shu-qing DU Kai LIU Qin ZHOU Yan-qin LIU En-yong YAO Bao-an ZHAO Jun-long |
| Affiliation: | 1. National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China ; 2. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070,China;3. Shanghai Export and Import Inspection and Quarantine Bureau, Shanghai 200135,China;4. Hubei Veterinary Prevention and Control Station, Wuhan 430064,China;5. Research Institute of Animal Science and Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China |
| Abstract: | The complete 16S rRNA gene of E.wenyonii-Hubei strain was determined by PCR.It was sequenced and blasted.The results showed that the sequence was 98.7% identical to that of Neimark published sequence(AF016546).The 1 471bp complete sequence was compared with the sequence of 5 mycoplasmas,14 haemotrophic bacteria and Anaplasma marginale available in GenBank.The data were analyzed and a phylogenetic tree was established.The results indicated that the hereditary distance of E.wenyonii was close to that of pneumoniae group,and the hereditary distance was far from Anaplasma marginale. |
| Keywords: | Eperythrozoon wenyonii 16S rRNA PCR, sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |