^14C—甲磺隆的鉴定方法研究

采用反相高效液相色谱法（HPLC）测定了^14C-甲磺隆的化学纯度，并分别用高效液相色谱与液体闪烁测量仪联用法（HPLC－LSC）以及薄层层析－同位素成像分析法（TLC－Isotope-imaging analysis,TLC-IIA)测定了^14C-甲磺隆的放射化学纯度。实验结果显示，合成的^14C-甲磺隆的化学纯度为97．3％，HPLC－LSC和TLC－IIA法测到的放射化学纯度分别为96．3％、96．5％和97．1％，所得结果一致。合成的^14C-甲磺隆的化学结构用液相色谱－质谱（LC－MS）法进行了验证，结果表明，该^14C-甲磺隆样品与甲磺隆标样的HPLC保留时间相同，LC－MS的总成或负分子离子峰和选择离子检测推断的分子量均为381，与甲磺隆的分子量相同。

Study on identification method of ~(14)C- metsulfuron methyl

The chemical purity of 14 C metsulfuron methyl was determined by high performance liquid chromatography (HPLC), its radiochemical purity was measured by HPLC LSC (Liquid Scintillation Counter) and TLC isotope imaging analysis (TLC IIA), respectively The results showed that the chemical purity of synthetic 14 C metsulfuron was 97 3% , and that its radiochemical purity determined by HPLC LSC and TLC IIA were 97 1% and 96 3% 96 5% , respectively The result identified by LC MS showed that the retention time of synthetic 14 C metsulfuron methyl was consistent with that of its standard sample, and that the molecular weight measured by MS(LC/MS total ion and selected ion monitoring) was 381 that is equal to MW of metsulfuron methyl