| 丝胶蛋白抗氧化肽的酶法制备及功能评价 |
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| 引用本文： | 范金波,任发政,孙 雁,王 芳,姜 鹭.丝胶蛋白抗氧化肽的酶法制备及功能评价[J].农业工程学报,2008,24(11):279-283. |
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| 作者姓名： | 范金波 任发政 孙 雁 王 芳 姜 鹭 |
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| 作者单位： | 中国农业大学食品科学与营养工程学院,教育部北京市功能乳品重点实验室,北京,100083 |
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| 摘 要： | 为进一步开发利用废蚕丝,充分挖掘丝胶蛋白的可利用程度,该文研究了6种蛋白酶水解制备丝胶肽及产品的抗氧化活性。筛选出Alcalase碱性蛋白酶为制备丝胶抗氧化肽的理想蛋白酶,并以ABTS体系评价了丝胶肽的抗氧化活性。通过单因素试验研究了反应时间、底物浓度、反应温度、pH值、酶底物比对酶水解制备丝胶肽的影响;并通过回归正交旋转试验设计对水解条件进行优化,建立各因素与水解度和抗氧化活性的数学模型,确定Alcalase酶水解丝胶蛋白的最佳水解条件为:反应时间240min,底物浓度2%,反应温度55℃,pH8.5,酶底物比2.375%。最佳水解条件下水解度为33.35%,ABTS清除活性为43.19%。该研究为开发丝胶抗氧化肽功能产品提供了技术依据。
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| 关 键 词： | 蛋白水解 水解 优化 丝胶 Alcalase 抗氧化活性 ABTS |
| 收稿时间： | 3/6/2008 12:00:00 AM |
| 修稿时间： | 2008/6/19 0:00:00 |
Enzymatic preparation and functional evaluation of antioxidant peptides derived from silk sericin |
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| Fan Jinbo,Ren Fazhen,Sun Yan,Wang Fang and Jiang Lu.Enzymatic preparation and functional evaluation of antioxidant peptides derived from silk sericin[J].Transactions of the Chinese Society of Agricultural Engineering,2008,24(11):279-283. |
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| Authors: | Fan Jinbo Ren Fazhen Sun Yan Wang Fang and Jiang Lu |
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| Institution: | College of Food Science and Nutritional Engineering,China Agricultural University,The Key Laboratory of Functional Dairy of Ministry of Education and Beijing,Beijing 100083,China,College of Food Science and Nutritional Engineering,China Agricultural University,The Key Laboratory of Functional Dairy of Ministry of Education and Beijing,Beijing 100083,China,College of Food Science and Nutritional Engineering,China Agricultural University,The Key Laboratory of Functional Dairy of Ministry of Education and Beijing,Beijing 100083,China,College of Food Science and Nutritional Engineering,China Agricultural University,The Key Laboratory of Functional Dairy of Ministry of Education and Beijing,Beijing 100083,China and College of Food Science and Nutritional Engineering,China Agricultural University,The Key Laboratory of Functional Dairy of Ministry of Education and Beijing,Beijing 100083,China |
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| Abstract: | |
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| Keywords: | proteolysis hydrolysis optimization silk sericin Alcalase antioxidant activities ABTS |
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