| 药用植物牛大力组织培养初探 |
| |
| 引用本文： | 潘颖南,张向军,蒙平,庾韦花,陈少珍,唐军,黄素梅,邹瑜.药用植物牛大力组织培养初探[J].广西农业科学,2010,41(6):523-525. |
| |
| 作者姓名： | 潘颖南 张向军 蒙平 庾韦花 陈少珍 唐军 黄素梅 邹瑜 |
| |
| 作者单位： | 广西农业科学院生物技术研究所,南宁,530007 |
| |
| 基金项目： | 广西农业科学院科技发展基金项目 |
| |
| 摘 要： | 以牛大力幼嫩茎段为外植体,采用0.1%HgCl2不同处理时间对外植体进行灭菌;以MS为基本培养基,探讨不同浓度6-BA（2.0、2.5 mg/L）和NAA（0.2、1.0 mg/L）对无菌外植体愈伤组织和芽的诱导效果;以1/2MS为生根培养基,分别附加不同浓度NAA（0～2.5 mg/L）和IBA（0～2.5 mg/L）,探讨对生根培养的效果。结果表明,0.1%HgCl2处理15 min,外植体褐化率和污染率较低;MS培养基中添加不同浓度6-BA和NAA组合均能诱导牛大力茎段产生愈伤组织,但以添加1.0mg/L NAA的愈伤组织诱导率最高;添加NAA和6-BA可诱导牛大力茎段上的腋芽萌动再生,但最高出芽率仅78.6%;再生芽的适宜增殖培养基为MS＋2.0 mg/L 6-BA＋0.05 mg/L NAA,培养30 d,增殖倍数可达4～5倍;在培养基1/2MS＋NAA 2.5mg/L＋IBA 2.5 mg/L中培养,其生根率可达66.7%;经过炼苗,移栽到菜园土和河沙（3∶1）的混合基质中,30 d后试管苗的移栽成活率可达85%以上。
|
| 关 键 词： | 牛大力 茎段 愈伤组织 诱导 生根 |
Tissue culture of medicinal plant Millettia speciosa Champ. |
| |
| PAN Ying-nan,ZHANG Xiang-jun,MENG Ping,YU Wei-hua,CHEN Shao-zhen,TANG Jun,HUANG Su-mei,ZOU Yu.Tissue culture of medicinal plant Millettia speciosa Champ.[J].Guangxi Agricultural Sciences,2010,41(6):523-525. |
| |
| Authors: | PAN Ying-nan ZHANG Xiang-jun MENG Ping YU Wei-hua CHEN Shao-zhen TANG Jun HUANG Su-mei ZOU Yu |
| |
| Institution: | PAN Ying-nan,ZHANG Xiang-jun,MENG Ping,YU Wei-hua,CHEN Shao-zhen,TANG Jun,HUANG Su-mei,ZOU Yu(Biotechnology Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China) |
| |
| Abstract: | The young stems of Millettia speciosa Champ.were used as explants.After sterilization with 0.1% HgCl2 for 12,15 and 18 min,the callus and buds of explants were induced in MS medium added with different concentrations of 6-BA(2.0,2.5 mg/L)and NAA(0.2,1.0 mg/L).The 1/2MS medium added with NAA(0-2.5 mg/L) and IBA(0-2.5 mg/L) was used to conduct root culture in induced plantlets.The results showed that the browning and infection rate of explants were lower when sterilized for 15 min by 0.1% HgCl2.The callus of ... |
| |
| Keywords: | Millettia speciosa Champ stem callus induction rooting |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
