板栗壳中多酚的提取及体外抗氧化性研究

研究了不同因素对板栗壳多酚提取效果的影响,并对不同条件下板栗壳多酚的抗氧化性进行了研究。结果表明,板栗壳多酚的最佳提取条件为:乙醇体积分数59.7%,料液比1∶18(g∶mL),浸提温度52.6℃,浸提次数3次。在此最优工艺条件下板栗壳多酚提取得率预测值为76.0 mg/g,实验值75.9 mg/g。板栗壳多酚具有较强的清除自由基能力,在25~200 mg/L范围内,随质量浓度升高其还原能力和清除二苯基苦基肼自由基(DPPH.)、羟基自由基(.OH)、超氧阴离子自由基(O-2.)的能力逐渐增强,当200 mg/L时其还原能力达0.841,DPPH.抑制率达89.7%,均高于同质量浓度的2,6二-叔丁基对甲酚(BHT),但低于同质量浓度Vc,对O-2.和.OH的清除率分别为93.2%和94.0%,均高于同质量浓度BHT和Vc。

Study on Extraction and Antioxidation Property of Polyphenol from Chestnut Shells

The effects of different factors on extraction of polyphenol from chestnut shell were studied, and the antioxidation pro-perty of polyphenol of chestnut shell was also studied. The results showed that the optimum extraction conditions were:ethanol volume fraction 59.7%, solid-liquid ratio 1:18(g:mL), extraction temperature 52.6℃ and extract 3 times. The predicted yield of chestnut shell polyphenol is 76.0 mg/g and the test result is 75.9mg/g. Polyphenol in chestnut shell has strong ability to eliminate free radicals. The abilities of reducing and removing DPPH·,·OH, O_2~-·gradually increased with the increase of concentration in the range of 25 to 200mg/L. The ability of reduction reached 0.841, and the rate of inhibition reached 89.7% at 200mg/L, which are higher than those of 2,6-di-tert-butyl-p-cresol (BHT) but lower than those of V_C, at similar mass concentration. The removal rates of O_2~- ·and ·OH are 93.2% and 94.0%, respectively, at concentration of 200mg/L, which are higher than BHT and V_C.