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| 正交设计优化花菜类SRAP分子标记体系的研究 | |
| 引用本文： | 李媛,姚雪琴,谢祝捷,龚义勤,柳李旺. 正交设计优化花菜类SRAP分子标记体系的研究[J]. 上海农业学报, 2010, 26(1): 42-45 |
| 作者姓名： | 李媛 姚雪琴 谢祝捷 龚义勤 柳李旺 |
| 作者单位： | 1. 上海市农业科学院园艺研究所,上海市设施园艺技术重点实验室,上海,201106;南京农业大学园艺学院,南京,210095 2. 上海市农业科学院园艺研究所,上海市设施园艺技术重点实验室,上海,201106 3. 南京农业大学园艺学院,南京,210095 |
| 基金项目： | 上海市科委科技攻关计划 |
| 摘 要： | 采用正交设计，从Mg^2＋、dNTPs、引物三种因素3个水平对青花菜SRAP反应体系进行优化，确立适合青花菜的SRAP反应体系，并在6个青花菜和花椰菜品种中进行验证。结果表明：20μ/L反应体系中适宜体系的浓度dNTPs为0．2mmol／L、Mg^2＋为1．25mmol／L、上下引物各0．4μmol／L、DNA为20ng、Taq酶1．2U、退火温度为50℃。 |
| 关 键 词： | 青花菜 花椰菜 SRAP 体系优化 |
Optimization of the SRAP reaction system in broccoli and cauliflower by orthogonal design |
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| LI Yuan,YAO Xue-qin,XIE Zhu-jie,GONG Yi-qin,LIU Li-wang. Optimization of the SRAP reaction system in broccoli and cauliflower by orthogonal design[J]. Acta Agriculturae Shanghai, 2010, 26(1): 42-45 | |
| Authors: | LI Yuan YAO Xue-qin XIE Zhu-jie GONG Yi-qin LIU Li-wang |
| Affiliation: | LI Yuan1,2,YAO Xue-qin1,XIE Zhu-jie1,GONG Yi-qin2,LIU Li-wang2(1Horticultural Research Institute,SAAS,Shanghai Key Laboratory of Protected HorticulturalTechnology,Shanghai 201106,China,2College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China) |
| Abstract: | By an orthogonal design the reaction system of brocco's SRAP(sequence-related amplified polymorphism)was optimized in terms of Mg~(2+),dNTPs and primers three factors and their three levels,and then the optimized SRAP reaction system was verified with 6 broccoli and cauliflower varieties.The result indicated that in a 20 μL reaction system the appropriate concentrations of dNTPs,Mg~(2+),and forward and reverse primers were respectively 0.2 mmol/L,1.25 mmol/L,0.4 μmol/L and 0.4 μmol/L,the DNA of template was 20 ng,and Taq DNA polymerase 1.2 U,the best annealing temperature was 50 ℃.The results offer the technique basis of using SRAP technology to study broccoli germplasm resource. |
| Keywords: | SRAP Broccoli Cauliflower SRAP(Sequence-related amplified polymorphism) System optimization |
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