| 节瓜基因组AFLP体系建立的研究 |
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| 引用本文： | 于远,程志学,陈清华,黄河勋,林毓娥,梁肇均. 节瓜基因组AFLP体系建立的研究[J]. 广东农业科学, 2008, 0(7): 42-45 |
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| 作者姓名： | 于远 程志学 陈清华 黄河勋 林毓娥 梁肇均 |
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| 作者单位： | 广东省农科院蔬菜研究所/广东省果蔬新技术重点实验室,广东,广州,510640;华南农业大学园艺学院,广东,广州,510640 |
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| 基金项目： | 广东省科技攻关计划 , 广东省自然科学基金 |
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| 摘 要： | 以节瓜雌性系K36和强雄系G为材料,从DNA提取、酶切连接、预扩和选扩等方面进行选择优化,建立高效稳定的AFLP分析体系,同时从64对AFLP引物组合筛选出多态性好的引物组合6对,并以该体系开展节瓜雌性性状分子标记和图谱构建等遗传分析研究。
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| 关 键 词： | 节瓜 雌性 DNA AFLP |
Establishment of AFLP analysis system in Chieh-qua |
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| YU Yuan,CHENG Zhi-xue,CHEN Qing-hua,HUANG He-xun,LIN Yue,LIANG Zhao-jun. Establishment of AFLP analysis system in Chieh-qua[J]. Guangdong Agricultural Sciences, 2008, 0(7): 42-45 |
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| Authors: | YU Yuan CHENG Zhi-xue CHEN Qing-hua HUANG He-xun LIN Yue LIANG Zhao-jun |
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| Abstract: | An efficient and stable AFLP analysis system was established for the Chieh-qua gynoecious line K36 and strong male line G,which was done by selection and optimization in the aspects of DNA extraction,enzyme digestion and ligation,preamplification and selective amplification.6 pairs primer combinations with good polymorphism were selected from 64 pairs AFLP primer combinations.The AFLP analysis system established was being used for genetic analysis such as molecular marker of gynoecious genes and construction of linkage map,and so on. |
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| Keywords: | Chieh-qua gynoecious DNA AFLP |
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