烟草种子不同成熟时期形态特征的激光扫描共聚焦显微镜观察

 对烤烟K326不同成熟时期的种子进行了激光扫描共聚焦显微镜（LSCM）观察，研究了烟草种子不同成熟时期形态、颜色，自发荧光强度的变化规律。发现烟草种子表面波状花纹在不同成熟时期均较稳定，各成熟时期种子的自发荧光强度存在显著差异。结果表明应用LSCM进行烟草种子检测可作为烟草种子质量控制以及种质资源研究的依据之一。

Observation on Morphorus of the Different Mature Stages of Tobacco Seed with Laser Scanning Confocal Microscope

The morphorus, color and auto fluoresent intensity of the flue cured tobacco K326 seeds at different mature stage are studied by Laser scanning confocal microscope. It shows that the corrugations on the surface of the seeds at different mature stage are stable and the auto fluoresent intensity is significantly different. The results indicated the application of LSCM to the observation of tobacco seeds can be used to germplasm research and quality supervision of tobacco seeds.