| 玉米抗草甘膦基因(sxglr-11)的构建与表达 |
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| 引用本文： | 王艳梅,王陆军,白建荣,赵明.玉米抗草甘膦基因(sxglr-11)的构建与表达[J].山西农业科学,2011,39(5):389-391,407. |
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| 作者姓名： | 王艳梅 王陆军 白建荣 赵明 |
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| 作者单位： | 1. 山西省农业科学院作物科学研究所,山西,太原,030032
2. 山西省农业科学院,山西,太原,030006 |
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| 基金项目： | 山西省青年基金，山西省农业科学院科技攻关项目 |
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| 摘 要： | 将现有的抗草甘膦基因(sxglr-11)和带有Ubiquitin启动子的载体进行连接,用壮观霉素(spectino-mycin)抗生素标记,构建携带抗草甘膦基因(sxglr-11)的大肠杆菌。然后利用三亲杂交法,将构建的真核表达载体转入根瘤农杆菌菌株LBA4404。对菌落进行PCR检测以及质粒双酶切产物进行电泳检测,并用构建的工程菌转化玉米试管苗。研究结果表明,表达载体成功转入根瘤农杆菌LBA4404。
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| 关 键 词： | 玉米 真核表达载体 根癌农杆菌 抗草甘膦基因 |
Construction and Expression of Anti-Glyphasate Gene(sxglr-11) |
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| WANG Yan-mei,WANG Lu-jun,BAI Jian-rong,ZHAO Ming.Construction and Expression of Anti-Glyphasate Gene(sxglr-11)[J].Journal of Shanxi Agricultural Sciences,2011,39(5):389-391,407. |
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| Authors: | WANG Yan-mei WANG Lu-jun BAI Jian-rong ZHAO Ming |
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| Institution: | WANG Yan-mei1,WANG Lu-jun1,BAI Jian-rong1,ZHAO Ming2(1.Institute of Crop Sciences,Shanxi Academy of Agricultural Sciences,Taiyuan 030032,China,2.Shanxi Academy of Agricultural Sciences,Taiyuan 030006,China) |
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| Abstract: | Glyphosate-resistant gene existing （sxglr-11 ）and vector carrying the Uhiquitin promoter were connected and marked with speetinomycin （spectinomycin） antibiotic marker, to construct gene carrying glyphosate （ sxglr- 11 ） in the coliform bacilli. Later, with three-parental mating method, the eukaryotic expression vector transferred into Agrobacterium tumefaciens strain LBA4404. The products of mycelium PCR, plasmid PCR and plasmid digested by EcoR I and BamH I enzyme were tested by electrophoresis, and the constructed project Agrobacterium was cultured with plantlet of maize. The result showed that eukaryotic expression vector had been transformed into A grob acte rium tumefacie ns LBA4404. |
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| Keywords: | maize eukaryotic expression vector Agrobacterium tumefaciens anti-glyphasate gene |
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