| 黄绿木霉菌菌株产几丁质酶发酵条件的研究 |
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| 引用本文： | 林志伟,张海燕,孙冬梅,秦翠红,南大鹏.黄绿木霉菌菌株产几丁质酶发酵条件的研究[J].安徽农业科学,2011,39(9):5043-5044. |
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| 作者姓名： | 林志伟 张海燕 孙冬梅 秦翠红 南大鹏 |
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| 作者单位： | 黑龙江八一农垦大学农学院,黑龙江大庆,163319;河南大学生命科学学院,河南开封,475001 |
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| 基金项目： | 河南大学校内科学研究基金(2008YBGG043) |
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| 摘 要： | 目的]研究黄绿木霉菌（Trichoderma aureoviride sp.）产几丁质酶的最佳发酵条件,为几丁质酶工业提供新酶源。方法]采用正交试验设计,以DNS法测定酶解后糖含量变化为指标,对发酵条件进行优化。结果]在以2%胶体几丁质为碳源、2%蛋白胨为氮源、摇床转速为170 r/min时,黄绿木霉菌产几丁质酶的最佳发酵条件组合：培养基的初始pH为5,接种量为8%,瓶装量为20 ml,在28℃下培养6 d。结论]试验确定了黄绿木霉菌产几丁质酶最佳条件,为其在工业生产中的应用奠定了基础。
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| 关 键 词： | 黄绿木霉 几丁质酶 发酵条件 |
Study on the Fermentation Conditions of Trichoderma aureoviride sp. for the Production of Chitinase |
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| LIN Zhi-wei et al.Study on the Fermentation Conditions of Trichoderma aureoviride sp. for the Production of Chitinase[J].Journal of Anhui Agricultural Sciences,2011,39(9):5043-5044. |
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| Authors: | LIN Zhi-wei |
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| Institution: | LIN Zhi-wei et al(Heilongjiang Bayi Agriculture University,Daqing,Heilongjiang 163319) |
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| Abstract: | Objective] The paper was to study the best fermentation conditions of Trichoderma aureoviride sp.for the production of chitinase,thus provide new enzyme source for citinase industry.Method] Using orthogonal experimental design,with the variation of sugar content after enzymatic hydrolysis measured by DNS method as the indicator,the fermentation conditions were optimized.Result] Taking colloidal chitin as the carbon source and 2% peptone as the nitrogen source with the shaking speed of 170 r/ min,the opti... |
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| Keywords: | Trichoderma aureoviride sp Chitinase Fermentation condition |
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