| 快速小量提取小麦叶片DNA的一种简易方法 |
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| 引用本文： | 王敏,那冬晨,姬虎太,张定一. 快速小量提取小麦叶片DNA的一种简易方法[J]. 安徽农业科学, 2009, 37(35): 17384-17385 |
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| 作者姓名： | 王敏 那冬晨 姬虎太 张定一 |
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| 作者单位： | 山西省农业科学院小麦研究所,山西临汾,041000;山西师范大学生命科学院,山西临汾,041000 |
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| 基金项目： | 国家转基因生物新品种培育重大专项"抗逆转基因小麦新品种培育"重大课题，国家科技支撑计划课题，国家现代小麦产业技术体系山西综合试验站;国家公益性行业(农业)科研专项(山西省) |
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| 摘 要： | [目的]寻求少量、快速提取小麦幼叶DNA的方法。[方法]以小麦幼叶为试验材料，用改良的CTAB法小量快速提取转基因小麦总DNA。所提取的DNA以0．8％琼脂糖凝胶电泳检测。[结果]改良的CTAB法提取小麦基因组DNA所提取的DNA纯度高、无降解现象，适用于进行常规PCR扩增。[结论]该研究为小麦DNA的提取提供了一种所需材料量少、简便、快速的方法。
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| 关 键 词： | 小麦 DNA提取 简便快速 |
A Simple and Quick Method of Extracting Genomic DNA from Wheat Leaf |
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| WANG Min et al. A Simple and Quick Method of Extracting Genomic DNA from Wheat Leaf[J]. Journal of Anhui Agricultural Sciences, 2009, 37(35): 17384-17385 |
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| Authors: | WANG Min et al |
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| Affiliation: | WANG Min et al(Wheat Research Institute,Shanxi Academy of Agricultural Sciences,Linfen,Shanxi 041000) |
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| Abstract: | [Objective] The aim was to seek for a simple and quick method of extracting genomic DNA from wheat leaf.[Method] Taking young leaves of wheat as test material,total DNA of transgenic wheat was extracted by using modified CTAB method.Extracted DNA was detected by 0.8% agarose gel electrophoresis.[Result] DNA purity of extracted genome DNA from wheat was high and no degradation phenomenon using modified CTAB method,and was suitable for carrying out normal PCR amplification.[Conclusion] This study provides a s... |
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| Keywords: | Wheat DNA extraction Simple and quick |
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