| 表达H5N1亚型禽流感HA-NA基因重组火鸡疱疹病毒的构建 |
| |
| 引用本文： | 赵冬凤,高轩,刘新文,胡潇,李明义.表达H5N1亚型禽流感HA-NA基因重组火鸡疱疹病毒的构建[J].中国动物检疫,2008,25(4):20-22. |
| |
| 作者姓名： | 赵冬凤 高轩 刘新文 胡潇 李明义 |
| |
| 作者单位： | 1. 河北农业大学,071001
2. 青岛易邦生物工程有限公司 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| |
| 摘 要： | 将禽流感病毒H5N1亚型具有免疫原性的血凝素(HA)基因和神经氨酸酶(NA)基因,克隆于表达质粒Tmd-CMV-IRES-EGFP中,使其位于含细胞巨化病毒(CMV)早期启动子的调控下,并与增强型绿色荧光蛋白基因(EGFP)串联,将上述串联基因插入到火鸡疱疹病毒(HVT)基因的非必需区(US10)中,从而构建了带标记的重组转移质粒。将该转移载体质粒感染HVT的鸡胚成纤维细胞(CEF),利用脂质体共同转染CEF,待病毒蚀斑出现后,利用蚀斑筛选带有绿色荧光的重组病毒蚀斑,数轮蚀斑纯化,经荧光和PCR初步鉴定获得了重组火鸡疱疹病毒。
|
| 关 键 词： | 禽流感病毒 HA-NA基因 重组火鸡疱疹病病毒 |
| 文章编号： | 1005-944X(2008)04-0020-03 |
Construction of a Recombinant Turkey Herpesvirus Virus Expressing HA and NA Gene of Avian Influenza Virus |
| |
| Zhao Dongfeng,Gao Xuan,Liu Xinwen,Hu Xiao,Li Mingyi.Construction of a Recombinant Turkey Herpesvirus Virus Expressing HA and NA Gene of Avian Influenza Virus[J].China Journal Of Animal Quarantine,2008,25(4):20-22. |
| |
| Authors: | Zhao Dongfeng Gao Xuan Liu Xinwen Hu Xiao Li Mingyi |
| |
| Institution: | Zhao DongFeng,Gao Xuan,Liu XinWen etal |
| |
| Abstract: | Haemagglutinin(HA) and neuraminidase (NA) genes of AIV were cloned in Tmd-CMV-IRES-EGFP. CMV-HA-IRES-NA-EGFP were inserted into the US10 gene region of herpesvirus of turkey(HVT).Then the transfer vector plasmid and HVT DNA were cotransferred onto CEF cells. When virus plaque appeared three days later,the green fluorescent plaque was seen and picked. The recombinant turkey herpesvirus virus was purified by several cycles of plaque screening. By green fluorescence and PCR,the recombinant HVT virus was identified. |
| |
| Keywords: | AIV HA and NA genes Recombinant turkey herpesvirus Virus |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国动物检疫》浏览原始摘要信息 |
| 点击此处可从《中国动物检疫》下载免费的PDF全文 |
|
