| 茄科蔬菜立枯丝核菌的融合群鉴定 |
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| 引用本文： | 伍恩宇,XIA Hai-bo,于金凤.茄科蔬菜立枯丝核菌的融合群鉴定[J].植物病理学报,2008,38(4):429-432. |
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| 作者姓名： | 伍恩宇 XIA Hai-bo 于金凤 |
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| 作者单位： | 山东农业大学植物保护学院植物病理学系, 泰安 271018 |
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| 基金项目： | 山东省优秀中青年科学家研究奖励基金，山东农业大学校科研和教改项目 |
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| 摘 要： | Sixty-five samples were collected from rhizosphere soil, hot pepper and tomato plants showing damping-off, root rot and stem rot in Taian, Shouguang of Shandong province and Zhouzhi, Taibai of Shaanxi province. Thirty-nine Rhizoctonia solani isolates were obtained from these samples. The results of anastomosis group (AG) identification and sequence analysis of 5.8S rDNA-ITS of the isolates showed that thirty-six isolates (92.3%) belonged to AG-4, while only three (7.7%) belonged to AG-5. The isolates of AG4 could further be divided into two subgroups of AG4-HG-Ⅰ and AG4-HG-Ⅲ. The 5.8S rDNA-ITS sequences of the selected isolates of the two subgroups had the 99%-100% identity with standard isolates of AG4-HG-Ⅰ and AG4-HG-Ⅲ (from GenBank). Among the analyzed isolates, AG4-HG-Ⅰ subgroup was the dominant with the frequency of 79.5%. Subgroup AG-4-HG-Ⅲ with the frequency of 12.8% was the second. This is the first report that subgroup AG4-HG-Ⅲ of R. solani isolated from Solanaceae vegetable crops in China.
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| 关 键 词： | 立枯丝核菌 茄科蔬菜 鉴定 Rhizoctonia 融合群 苗期立枯病 病原学 根腐病 |
Anastomosis group identification of Rhizoctonia solani isolated from Solanaceae vegetable crops |
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| WU En-yu,XIA Hai-bo,YU Jin-feng.Anastomosis group identification of Rhizoctonia solani isolated from Solanaceae vegetable crops[J].Acta Phytopathologica Sinica,2008,38(4):429-432. |
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| Authors: | WU En-yu XIA Hai-bo YU Jin-feng |
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| Institution: | Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Taian 271018, China |
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| Abstract: | Sixty-five samples were collected from rhizosphere soil, hot pepper and tomato plants showing damping-off, root rot and stem rot in Taian, Shouguang of Shandong province and Zhouzhi, Taibai of Shaanxi province. Thirty-nine Rhizoctonia solani isolates were obtained from these samples. The results of anastomosis group (AG) identification and sequence analysis of 5.8S rDNA-ITS of the isolates showed that thirty-six isolates (92.3%) belonged to AG-4, while only three (7.7%) belonged to AG-5. The isolates of AG4 could further be divided into two subgroups of AG4-HG-Ⅰ and AG4-HG-Ⅲ. The 5.8S rDNA-ITS sequences of the selected isolates of the two subgroups had the 99%-100% identity with standard isolates of AG4-HG-Ⅰ and AG4-HG-Ⅲ (from GenBank). Among the analyzed isolates, AG4-HG-Ⅰ subgroup was the dominant with the frequency of 79.5%. Subgroup AG-4-HG-Ⅲ with the frequency of 12.8% was the second. This is the first report that subgroup AG4-HG-Ⅲ of R. solani isolated from Solanaceae vegetable crops in China. |
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| Keywords: | tomato hot pepper Rhizoctonia solani AG-4-HG-Ⅲ rDNA-ITS |
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