利用野生大豆染色体片段代换系定位单株粒重QTL

以野生大豆ZYD00006为供体亲本,黑龙江省主栽品种绥农14为轮回亲本,连续多年回交并自交,构建了高世代染色体片段代换系BC3F3代161个株行。该群体经多代回交的遗传背景相对一致,大大提高了QTL定位的准确度。结合单因素方差分析法和独立样本T检验法对群体进行QTL定位,共获得9个单株粒重的QTL,分布于7个连锁群。两种方法中均被检测到的有3个QTL,分别为QSW-J-1、QSW-J-2和QSW-G-1;QSW-G-1和QSW-G-2与已有研究结果相吻合;其余7个QTL为新发现QTL,可能是本材料特有位点;其中QSW-J-1的导入片段长度是7.0 c M,且加性效应值为-2.7 g,可作为继续研究的首选位点。

QTL Mapping for Seed Weight per Plant by using the Wild Soybean Chromosome Segment Substitution Lines

To study the QTL mapping of seed weight per plant,which is beneficial to increase the yield of soybean.In this study,the BC3 F3 population is a chromosome segment substitution lines which constituted by 161 lines,with the wild soybean ZYD00006 as donor parent and the cultivar Suinong 14 as recurrent parent,selfing and backcross continuously for several years.The genetic background of this population relatively consistent,which reducing the interference greatly and improving the accuracy of QTL mapping.The QTL mapping was combined by ANOVA Method and T-test for independent samples.A total of 9 QTLs underlying seed weight per plant were detected which distributed on 7 linkage groups.Three QTLs were detected by both methods,including QSW-J-1,QSW-J-2,QSW-G-1.2 QTL were in accord with known results.Another 7 ones were the new discovery QTL,which should be specific loci in our materias.The fragment length of QSW-J-1 was 7.0 cM,and the additive effects of it is-2.7 g,which could be used as the first choice loci for further study.