棉花枯萎病菌同工酶电泳的研究

对棉花枯萎病菌的3、8和7号3个生理小种的标准菌株和新疆所采31个供试菌株采用聚丙烯酰胺凝胶电泳进行了酯酶同工酶谱、过氧化物同工酶谱和可溶性蛋白质谱分析.结果表明,各生理小种间的谱带存在明显差异,过氧化物同工酶出现的谱带数较少,酯酶同工酶谱和可溶性蛋白质谱谱带数较多,同为7号小种的不同菌株间其谱带虽表现出多型性,但其谱带一致性较高.据此可以看出,同工酶谱和可溶性蛋白质谱对棉花枯萎病菌生理小种的鉴定具有重要的参考价值,其中以酯酶同工酶谱和可溶性蛋白质谱分析为好.

Studies on the Isozymes Electrophoresis of Cotton-wilt Fusarium

The isozymes (Esterase, Peroxidase and soluble protein) of 31 isolates from Xinjiang and different standard physiologic races (3、7、8) were analysed with polyacrylamide gel electrophoresis. The result showed that the difference between physiologic races of cotton wilt fusarium was obvious, the bands of esterase isozyme and soluble protein were more than those of peroxidase isozyme. Although there were polymorph phenomena among different strains of the same 7 physiologic race, the identity of the bands was considerably high. So these identified that the isozymes Electrophoresis was of important consult value on identification of physiologic races of cotton-wilt fusarium ,especially esterase isozyme and soluble protein.