Effect of dietary lysine on growth,intestinal enzymes activities and antioxidant status of sub-adult grass carp (Ctenopharyngodon idella) |
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Authors: | Xue-Yin Li Ling Tang Kai Hu Yang Liu Wei-Dan Jiang Jun Jiang Pei Wu Gang-Fu Chen Shu-Hong Li Sheng-Yao Kuang Lin Feng Xiao-Qiu Zhou |
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Institution: | 1. Animal Nutrition Institute, Sichuan Agricultural University, Ya’an, 625014, Sichuan, China 2. Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Ya’an, 625014, Sichuan, China 3. Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Ya’an, 625014, China 4. Animal Nutrition Institute, Sichuan Academy of Animal Science, Chengdu, 610066, China
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Abstract: | The dietary lysine requirement of sub-adult grass carp (460 ± 1.5 g) was assessed by feeding diets supplemented with grade levels of lysine (6.6, 8.5, 10.8, 12.9, 15.0 and 16.7 g kg?1 diet) for 56 days. The test diets (28 % CP) contained fish meal, casein and gelatin as sources of intact protein, supplemented with crystalline amino acids. Weight gain (WG), feed intake and feed efficiency were significantly improved with increasing levels of lysine up to 12.9 g kg?1 diet and thereafter declined (P < 0.05). Quadratic regression analysis of WG at 95 % maximum response indicated lysine requirement was 10.9 g kg?1 diet. Activities of trypsin, chymotrypsin, lipase, Na+, K+-ATPase and alkaline phosphatase in intestine, creatine kinase activity in proximal and mid-intestine responded similar to WG (P < 0.05). In addition, lipid and protein oxidation decreased with increasing levels of lysine up to certain values and increased thereafter (P < 0.05); the anti-hydroxyl radical capacity, dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase (GST) activities and glutathione content were increased with increasing dietary lysine levels up to certain values in the detected tissues, except for hepatopancreatic GST. Requirement estimated on the basis of malondialdehyde content in intestine and hepatopancreas was 10.6 and 9.53 g lysine kg?1 diet, respectively. |
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