Effects of cold acclimation and storage temperature on crucian carp (Carassius auratus gibelio) in a waterless preservation

The research aims to explore the impact of cold acclimation and storage temperature on crucian carp in a waterless preservation. It is conducted by studying the influence of cold acclimation on crucian carp in temperatures of 5 and 1 °C h^?1, followed by having them preserved under waterless conditions at 4 and 0 °C for 24 h to analyze their aerobic and anaerobic capacities. The research findings revealed that the temperature drop at 1 °C h^?1 is conducive to preserving the activity of lactate dehydrogenase. The activity of isocitrate dehydrogenase was maintained, and the brain succinate dehydrogenase remained unchanged. With regards to alanine transaminase, its activity, being sensitive to the changes of storage temperatures, was maintained when the temperature was decreased to 0 °C and malondialdehyde was accumulated at the same temperature. Stored in cold environment, blood catalase was accumulated; however, obvious changes were not found in the liver. It is likely that cold acclimation contributes to retaining aerobic and anaerobic metabolism under waterless preservation as well as decreasing the damage of blood oxidation.