5-aza—dC联合TSA对不同供体细胞来源牛核移植胚胎体外发育的影响

为研究5-aza—dC和TSA提高核移植胚胎体外发育能力的作用是否有供体细胞特异性，实验选择皮肤成纤维细胞、胎儿成纤维细胞和卵丘颗粒细胞作为核供体细胞进行核移植，联合使用20nM（72h）5-aza—dc和50nM（12h）TSA处理供体细胞和重构胚，比较5-ttZa—dC＋TSA对不同供体细胞来源核移植胚胎体外发育的影响。实验结果表明，经5-azaLdC＋TSA处理后，极显著提高了3种供体细胞来源核移植胚胎的体外囊胚发育率（P〈0．01）。虽然胎儿成纤维细胞和卵丘颗粒细胞得到的发育率稍高于皮肤成纤维细胞组，但各对照组之间以及各处理组之间的体外发育率没有明显差异，5-aza—dC和TSA对核移植胚胎体外发育的影响没有供体细胞特异性。

Effects of 5-aza-dC and TSA on Development of Embryos Cloned from Different Donor Cells

To investigate the effects of 5-aza-dC and TSA on the development of embryos cloned from different donor cells,the fetal fibroblast cells,adult skin fibroblast cells,and cumulus cells were used as donor cells for nuclear transfer,the donor cells and cloned embryos were treated with TSA(50 nM,12 h) and 5-aza-dC(20 nM,72 h),and the effects of these reagents on the development of cloned embryos were investigated.The results show that 5-aza-dC combined with TSA could significantly promoted the development potential of cloned embryos (P<0.01),and the beneficial effects of TSA and 5-aza-dC on cloned embryos development were not of specific characteristics for the donor cells.