Interaction of NR2D subunit of NMDA receptor with MOCA |
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Authors: | BAI Ning YI Fei LIU Ting LI Xiao-man |
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Institution: | Institute of Translational Medicine, Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, Shenyang 110122, China |
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Abstract: | AIM: To identify the potential proteins interacting with NR2D subunit of NMDA receptor by yeast two-hybrid screening and to investigate the role of NR2D in excitotoxicity of the retina.METHODS: The Clontech GAL4 yeast two-hybrid system was used to screen the mouse brain cDNA library, and the bait plasmid containing C-terminus of NR2D was constructed. Physical interaction between 2 proteins was verified by co-immunoprecipitation assay. The subcellular localization of 2 proteins in the mouse retina was observed under microscope with immunofluorescence.RESULTS: Modifier of cell adhesion (MOCA) was identified as a new protein interacting with NR2D. MOCA and NR2D were co-expressed in the mouse retina. CONCLUSION: MOCA specifically interacts with NR2D, which provides the experimental basis for identifying the role of glutamate excitotoxicity in the retina neurodegeneration. |
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Keywords: | NR2D NMDA receptor Modifier of cell adhesion Yeast two-hybrid techniques Protein interaction |
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