Analysis on Polymorphisms of Three CNV Regions in Five Pig Breeds |
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Authors: | HUANG Shi-hui RAN Xue-qin WANG Jia-fu PAN Hua LI Rong-rong |
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Institution: | 1. College of Animal Science, Guizhou University, Guiyang 550025, China;2. Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China |
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Abstract: | The paper was aimed to investigate the polymorphism of copy number variation (CNV) in different pig breeds.Three CNV regions of CNVR91,CNVR92 and CNVR143 were chosen from the porcine SNP60 chip genotyping results.The polymorphisms of three CNVs were determined by Real-time quantitative PCR method,taking five pig breeds as samples,including Yorkshire pig,Xiang pig,Kele pig,Nuogu pig and Rongchang pig breeds.The results showed that the dominant status of CNVR91 was loss in Xiang pig,while it was normal in other four pig breeds.The major type of CNVR92 was deletion in Xiang pig,Yorkshire pig,Kele pig and Rongchang pig breeds,with a high normal percent in Nuogu pig.For CNVR143,the dominant event was gain in Xiang pig and Nuogu pig breeds,but it was not diverse in other three pig breeds.These results indicated that three CNV regions emerged with polymorphism in five pig breeds,which might have effects on gene expression in CNV regions and physiological function by dosage effect especially in Xiang pig,Nuogu pig and Kele pig breeds. |
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Keywords: | copy number variation pig breeds Real-time quantitative PCR polymorphism |
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