一个水稻卷叶突变体zw209的遗传分析与精细定位

叶片是植物光合作用的主要场所,优良的叶片形态有利于塑造理想株型,提高光合效率。为了研究叶片形态建成的分子机制,从水稻T-DNA突变体库中筛选到1个叶片极度卷曲的突变体zw209,突变体有2个显著特征:1突变体泡状细胞数量和面积均变小;2突变体的叶绿素含量较高,具有较高的光合效率。遗传分析表明,zw209的突变性状由一对隐性核基因控制。通过图位克隆,将基因(ZW209)精细定位到9号染色体长臂上,位于In Del136和In Del140之间的92.3 kb区域内。在这个区域内,尚未见报告已克隆的卷叶基因,很可能是一个新的基因位点。上述结果明确了突变体的表型特征及遗传规律,为克隆ZW209基因和揭示其作用机理奠定基础。

Genetic Analysis and Gene Mapping of a Rolled Leaf Mutant zw209 in Rice

Leaf is the main organ of plant photosynthesis, fine leaf shape is beneficial for shaping the ideal plant type and improve the efficiency of photosynthesis. In order to study the molecular mechanism of leaf morphogenesis, an extreme rolled leaf mutant zw209 was screened from the rice T-DNA insertion mutant library. Compared with the wild type, zw209 had two significant features: (1) the number and area of mutants bulliform cells were smaller; (2) the chlorophyll content and photosynthetic efficiency of mutant were higher. Genetic analysis showed that the mutant trait was controlled by a single recessive nuclear gene. Through map-based cloning, the gene ZW209 was located on the long arm of rice chromosome 9 and a 92.3 kb region between markers InDel136 and InDel140. There was no rolling leaf rice gene had been reported in this section, so the gene ZW209 might be a new gene loci. Above results defined the inheritance and phenotype characteristics of this inward rolled leaf mutant, which provided a basis for cloning this gene and functional mechanism analysis.