Analysis on Genetic Diversity and Phyletic Evolution of mtDNA Cytb Gene in Zhongdian Yak |
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Authors: | TU Shi-ying SONG Na-na CHAI Zhi-xin ZHONG Jin-cheng |
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Institution: | 1. Animal Health Supervision Authority of Yunnan, Kunming 650000, China;
2. Key Laboratory of Animal Genetics and Breeding of State Ethnic Affairs Commission and Ministry of Education, Southwest University for Nationalities, Chengdu 610041, China;
3. Institute of Tibetan Plateau Research, Southwest University for Nationalities, Chengdu 610041, China |
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Abstract: | In order to investigate the genetic diversity and the origin of evolutionary relationship of Zhongdian yak,we analyzed the complete sequence of 15 individuals Cytb gene,its sequence polymorphism was analyzed,and the phylogenetic tree was constructed.The results showed that the length of the nucleotide sequence were 1 140 bp,with nucleotide frequencies of 26.3%,31.8%,13.1% and 28.8% for T,A,G and C,respectively.Three haplotypes were identified of 15 individuals,with 3 polymorphic sites,including two conversions,one transversion,haplotype diversity was 0.2571 and nucleotide diversity was 0.00035.Phylogenetic analysis suggested that Zhongdian yak and Bos mutusc clustered firstly,then gathered with Bison bison,which indicated that there were high genetic similarity and closer genetic relationship,genetic similarity with other cattle genus was relatively low.Combining with the proof of molecular biology and paleontology,the result supported the point that Bos grunniens and Bos mutus were classified as an alone genus in Bovinae. |
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Keywords: | Zhongdian yak Cytb gene phylogenetic evolution genetic diversity |
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