Berberine promotes anti-proliferative effects of doxorubicin in T24 bladder cancer cells by enhancing apoptosis |
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Authors: | CHEN Qi-biao ZHAN Xiong-yu LV Xiu-xiu CHEN Bo QIN Xiao-ping CHEN Jian-fan HUANG Jun QU Li-jun ZHUO Yu-min |
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Institution: | 1. The First Affiliated Hospital, Jinan University, Guangzhou 510632, China;
2. Department of Pathophysiology, School of Medicine, Jinan University, Guangzhou 510632, China |
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Abstract: | AIM: To observe the effect of berberine (Ber) on doxorubicin (DOX)-induced apoptosis in bladder cancer T24 cells. METHODS: The cells were exposed to DOX in the presence or absence of different concentrations of Ber. The viability of the cells was determined by CCK-8 assay. The apoptosis was measured by Hoechst 33258 staining and the protein levels of cleaved caspase-3, cleaved caspase-9, Bcl-2 and Bax were detected by Western blotting.RESULTS: Ber enhanced the inhibitory effect of DOX on the viability of T24 cells and promoted DOX-induced apoptosis in T24 cells. DOX increased the protein levels of cleaved caspase-3, cleaved caspase-9 and Bax, all of which were enhanced by treatment with Ber. In contrast, Ber exposure further decreased the expression of Bcl-2 in DOX-treated T24 cells.CONCLUSION: Ber enhances the anti-proliferative effects of DOX through promoting apoptosis in bladder cancer cells. |
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Keywords: | Berberine Doxorubicin Bladder cancer T24 cells Apoptosis |
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