Effects of EGLN1 siRNA on growth of rat pulmonary artery smooth muscle cells under hypoxic condition |
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Authors: | SUN Li GA Qin YANG Quan-yu JIN Guo-en |
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Affiliation: | Research Center of High-Altitude Medicine, Qinghai University, Xining 810001, China |
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Abstract: | AIM: To observe whether EGLN1 gene is involved in the growth of pulmonary arterial smooth muscle cells (PASMCs) during hypoxia when EGLN1 gene expression was interference by siRNA. METHODS: The rat primary pulmonary arterial smooth muscle cells were cultured, and the specific lipidosome of EGLN1 siRNA was constructed and transfected into the PASMCs. The transfected PASMCs were cultured under hypoxia or normoxia conditions, respectively. The viability of the PASMCs was detected by CCK-8 assay. The protein expression of EGLN1 and vascular endothelial growth factor (VEGF) was determined by Western blot. RESULTS: The viability of the PASMCs was increased and the protein expression of VEGF was up-regulated in the PASMCs under hypoxic condition in a time-dependent manner. In hypoxia or normoxia condition, the viability and VEGF protein expression of the PASMCs were suppressed by EGLN1 siRNA. CONCLUSION: EGLN1 gene may involve in the growth of rat PASMCs by regulating VEGF protein level under hypoxic condition. |
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Keywords: | Hypoxia EGLN1 gene Rat pulmonary arterial smooth muscle cells Cell viability |
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