利用微卫星标记对宁强矮马和蒙古马遗传多样性的研究

通过8个微卫星位点的多态性检测对宁强矮马和蒙古马共83个个体进行了遗传多样性分析。计算各微卫星位点的等位基因频率、有效等位基因数(Ne)、多态信息含量(PIC)、杂合度(H)和F统计量。结果表明:8个微卫星座位中HMS2变异最大,HTG6变异最小;蒙古马的Ne、PIC、H的平均值均高于宁强马;总群体的平均遗传分化系数Fst为0.017(P<0.01),群体内近交系数Fis为0.171(P<0.01)。说明蒙古马和宁强矮马虽外形和性能上有很大差异,但在品种形成和进化上有着较近的遗传关系。

Study on the Genetic Diversity of Ningqiang Pony and Mongolian Horse Using Microsatellite Markers

The genetic diversity of 83 samples of two Chinese indigenous horse breeds, Ningqiang pony and Mongolian horse, was investigated with 8 microsatellites. Allele frequency, Ne, PIC, H, and F-statistic of each microsatellite locus were studied in the preseat study. The results showed that HMS2 and HTG6 demonstrated the highest and lowest polymorphisms among the 8 locus, respectively. The Ne, PIC and H of Mongolian horse were higher than those in Ningqiang pony, and the Fst of all studied horses was 0.017(P < 0.01), and Fis between two horses was 0.171(P < 0.01). The results suggested that Ningqiang pony and Mongolian horse had close genetic relationship though there were significantly different appearance characters between the two breeds.