| 甜菜碱的电喷雾质谱研究 |
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| 引用本文： | 王佳钰,张婷婷,许庆轩,孙晖,周芹,吴玉梅.甜菜碱的电喷雾质谱研究[J].中国糖料,2011(3). |
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| 作者姓名： | 王佳钰 张婷婷 许庆轩 孙晖 周芹 吴玉梅 |
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| 作者单位： | 农业部甜菜品质监督检验测试中心;黑龙江中医药大学药学院; |
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| 基金项目： | 黑龙江大学博士科研启动基金; 黑龙江省教育厅科学研究面上项目(11541278) |
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| 摘 要： | 使用电喷雾质谱检测了糖用甜菜中甜菜碱,并且采用串联质谱技术分析了甜菜碱的碎裂规律。实验结果表明:甜菜碱在酸性条件下检测,在质谱中表现为M+H]+离子,谱图比较简单,然而在中性条件下检测,簇和及加钠和加钾离子现象严重,谱图比较复杂,因此确定了甜菜碱的检测条件为酸性。在串联质谱中甜菜碱碎裂产生含氮和不含氮的两种特定的子离子,可以作为甜菜碱的定性依据。甜菜提取液不需经过复杂的前处理就可以检测出甜菜碱,操作简单、快速,灵敏度高、特异性好,可以作为复杂体系中甜菜碱的快速检测方法。
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| 关 键 词： | 甜菜碱 电喷雾质谱 电喷雾串联质谱 检测 |
Study on Betaine by Electrospray Ionization Mass Spectrometry |
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| WANG Jia-yu,ZHANG Ting-ting,XU Qing-xuan,SUN Hui,ZHOU Qin,WU Yu-mei.Study on Betaine by Electrospray Ionization Mass Spectrometry[J].Sugar Crops of China,2011(3). |
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| Authors: | WANG Jia-yu ZHANG Ting-ting XU Qing-xuan SUN Hui ZHOU Qin WU Yu-mei |
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| Institution: | WANG Jia-yu1,2,ZHANG Ting-ting1,XU Qing-xuan1,SUN Hui2,ZHOU Qin2,WU Yu-mei1(1.Center for the Control of Sugarbeet Quality,Ministry of Agriculture,P.R.China,Harbin 150080,China,2.Medicine College,Heilongjiang University of Chinese Medicine,Harbin 150040,China) |
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| Abstract: | Betaine is detected by using electrospray ionization mass spectrometry(ESI-MS) and tandem mass spectrometry(ESI-MSn).The analysis condition is optimized and its fragment mechanism also is concluded.The mass spectrum of betaine is very simple in acid solution,e.g.,only + peak is predominant.Therefore,there are more peaks of cluster ion and + and + in neutral solution,and the mass spectrum is rather complex,that is adverse to analysis.Thus,it is concluded that the optimum condition for detecting betaine is ac... |
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| Keywords: | betaine eletrospray ionization mass spectrometry(ESI-MS) eletrospray ionization tandem mass spectrometry(ESI-MSn) detection |
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