| 甜菜硝酸还原酶基因编码氨基酸偏好及功能预测分析 |
| |
| 引用本文： | 丁广洲,侯静,马凤鸣.甜菜硝酸还原酶基因编码氨基酸偏好及功能预测分析[J].中国糖料,2011(4):3-6. |
| |
| 作者姓名： | 丁广洲 侯静 马凤鸣 |
| |
| 作者单位： | 中国农业科学院甜菜研究所;黑龙江大学农作物研究院;东北农业大学农学院; |
| |
| 基金项目： | 国家自然科学基金(30571096)项目 |
| |
| 摘 要： | 利用同源序列克隆方法从标准偏高糖型甜菜二倍体纯系Ty7中获得氮素诱导甜菜硝酸还原酶基因片段,通过RACE技术克隆基因全长序列；该基因ORF长度2718 bp,编码905个氨基酸,分子量大小为102kD(ExPaSy的分子量分析),等电点为6.12,含有147bp的5'UTR和382 bp的3'UTR,Genbank上的...
|
| 关 键 词： | 甜菜 硝酸还原酶基因 氨基酸偏好 功能预测 |
Amino Acid Preferences and Analysis on Function Prediction of NR Gene in Sugarbeet(Beta vulgaris L.) |
| |
| DING Guang-zhou,,HOU Jing ,MA Feng-ming.Amino Acid Preferences and Analysis on Function Prediction of NR Gene in Sugarbeet(Beta vulgaris L.)[J].Sugar Crops of China,2011(4):3-6. |
| |
| Authors: | DING Guang-zhou HOU Jing MA Feng-ming |
| |
| Institution: | DING Guang-zhou1,2,3,HOU Jing 3,MA Feng-ming3(1.Sugarbeet Institute of Chinese Academy of Agricultural Sciences,Harbin 150080,China,2.Agricultural College of Heilongjiang University,3.Agronomy College of Northeast Agriculture University,Harbin 150030,China) |
| |
| Abstract: | From diploid sugarbeet line Ty 7,a cDNA clone related to nitrate reduction was isolated by Homology-based cloning.According to its sequences information,a novel full-length cDNA termed nitrate reductase gene(GenBank number:EU163265) was obtained by using rapid amplification of cDNA ends(RACE).it was 3247 bp long containing a 2718 bp ORF,flanked by a 147 bp 5'-UTR and a 328 bp 3'-UTR.It encoded 905 amino acids with a theoretical molecular weight of 102 kDa and an isoelectric point of 6.12.The result of the c... |
| |
| Keywords: | beet nitrate reductase gene amino acid preferences function prediction |
| 本文献已被 CNKI 等数据库收录！ |
|
