两性生殖卤虫16S rDNA分子系统学研究

目的]对两性生殖卤虫的分类地位和系统发育情况进行探讨。方法]测定了中国分布的A.sinicatibetiana、未定名的新疆鲸鱼湖卤虫和青海小柴旦卤虫3种两性生殖卤虫的16SrDNA序列,测序结果与Genbank已发表的另外11个两性生殖种卤虫的16SrDNA序列进行分析比较,并选取丰年虫科Artemiopsisstefanssoni为外群,运用MEGA软件中的NJ法构建分子系统树。结果]A.persimilis是卤虫属中原始的类群,A.franciscana和A.monica为进化类群,A.urmiana与A.s.sinica以及中国的其它种类有密切的亲缘关系。结论]基于线粒体16SrDNA序列的两性生殖卤虫系统发育关系为：A.persimilis→A.urmiana、A.sinica和A.tibetiana→A.tunisiana→A.monica→A.Franciscan.

Molecular Phylogeny of Bisexual Artemia Based on 16S rDNA

Objective] The research aimed at investigating the taxonomic position and phylogenetic relationship of bisexual brine shrimps.Method] 16S rDNA of three species of bisexual Artemia from China was determined;the homologous sequences between them and 11 relative species of Artemia from GenBank were compared;the molecular phylogenetic tree was constructed by Mega Microsoft using Artemiopsis stefanssoni as outgroup.Result] Artemia persimilis was the primal group in genus Artemia;Artemia franciscana and Artemia monica were the evolved groups;Artemia urmiana,Artemia sinica and other Artemia species from China shared a close genetic relationship.Conclusion] Based on the 16S rDNA sequence of them,the phylogenetic relationships of these bisexual Artemia species were A.persimilis→A.urmiana,A.sinica and A.tibetiana→A.tunisiana→A.monica→A.Franciscan.